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EC number: 232-430-1 | CAS number: 8027-33-6 A complex combination of organic alcohols obtained by the hydrolysis of lanolin.
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- From 12 June 2001 to 14 June 2001
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 001
- Report date:
- 2001
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method C.2 (Acute Toxicity for Daphnia)
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- 24-25th March 1997
Test material
- Reference substance name:
- Alcohols, lanolin
- EC Number:
- 232-430-1
- EC Name:
- Alcohols, lanolin
- Cas Number:
- 8027-33-6
- Molecular formula:
- UVCB
- IUPAC Name:
- Alcohols, lanolin
- Reference substance name:
- Lanolin Alcohols
- IUPAC Name:
- Lanolin Alcohols
- Details on test material:
- Sponsor's identification: Wollwachsalkohol I Lanolinalkohol
Description: Yellow, type wax
Batch number: Eucerit 6480
Active ingredient: Wool waxalcohol
Storage conditions: 7 ± 2 °C, protected from light and moisture
Composition: Aliphates: 25.5 %
Sterines: 60.6 %
Cholesterine: 31.2 %
CH-5dien-70ne: 8.9 %
Dehydrolanosterine: 5.5 %
Lanosterine: 10.9 %
Constituent 1
Constituent 2
Sampling and analysis
- Analytical monitoring:
- yes
- Details on sampling:
- - Concentrations:
Limit concentration: 1 : 1 = 0.13 mg DOC I L
- Sampling method: Not reported
- Sample storage conditions before analysis: Not reported
Test solutions
- Vehicle:
- yes
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION
The solution (1000 mg/L test item was weighed out) was prepared with dilution water one day prior to application and filtered (0.45 µm, single use)
Dispersion treatment: 24 h horizontal shaking
Control:
Dilution water without test item tested under same conditions as the test group.
Solubility of the test item:
The test item was clearly dissolved at the limit concentration.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name:
Water Flea
- Source:
Origin: Institut fOr Wasser-, Boden- und Lufthygiene des Bundesgesundheitsamtes, Corrensplatz, 14195 Berlin, FRG.
Breeder: DR.U.NoACK-LASORATORIUM, 31157 Sarstedt, FRG
- Age at study initiation:
Less than 24 h old
- Feeding during test:
Received no food during exposure
ACCLIMATION
- Acclimation period:
2 h
- Acclimation conditions:
2 h in dilution water
- Type and amount of food:
5 x weekly ad libitum with a mix of Scenedesmus subspicatus and ChIarella vulgaris, with an algae cell density of > 10E6 cells/mL.
- Health during acclimation:
No mortality observed
QUARANTINE (wild caught)
- Duration:
Not applicable
- Health/mortality:
Not applicable
Study design
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- yes
- Total exposure duration:
- 48 h
- Post exposure observation period:
- Not applicable
Test conditions
- Hardness:
- Total hardness: 263 mg CaCO3/L.
- Test temperature:
- 20 ± 2 °C constant
Measured using Hygro-thermograph (LUFFT). - pH:
- 7.64 - 7.82
The pH was measured using a pH-Meter 537 (WTW) - Dissolved oxygen:
- 8.06 mg/l - 8.61 mg/l
The dissolved oxygen concentration was measured using an Oximeter, Oxi 530 (WTW) - Salinity:
freshwater used.- Nominal and measured concentrations:
- Limit Concentration: 0.13 mg/l
- Details on test conditions:
- TEST SYSTEM
The definitive study was performed as limit test with the saturated solution as specified above under static conditions to enable the determination of immobilization after 24 and 48 hours.
- Test vessel:
Glass beakers (5 cm 10 x 8 cm H), 50 mL volume were used
- Type (delete if not applicable):
closed
- Material, size, headspace, fill volume:
20 mL
- Number of study organisms per concentration and control:
20 animals, divided into 4 parallel samples, each with 5 animals
-Number of animals per test vessel: 5
- Number of parallel samples per concentration: 4
OTHER TEST CONDITIONS
- Adjustment of pH:
Not reported
- Photoperiod:
16/8 h ligh/dark cycle
- Light intensity:
Diffuse light, illumination strength max. 20 µmol.m-2 . S·1 (corresponding to 1200 Ix) - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate
Results and discussion
Effect concentrations
- Duration:
- 48 h
- Dose descriptor:
- other: Limit concentration
- Effect conc.:
- 0.13 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: immobilisation
- Remarks on result:
- other: Not reported
- Details on results:
- - Behavioural abnormalities: Not recorded
- Other biological observations: None recorded
- Immobilisation of control:
The percentage immobility was determined in the tested limit concentration and the control after 24 hand 48 h. The immobilisation rates in % after 24 hand 48 h of exposure are given in Table 1.
There is no biologically significant effect neither in the tested limit concentration nor in control group.
- Other adverse effects control: No other effects observed.
- Abnormal responses: None recorded
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values:
The test item was clearly dissolved at the limit concentration. - Results with reference substance (positive control):
- - Results with reference substance valid?
Yes
- Mortality:
No mortalities recorded.
- EC50/LC50:
The percentage immobility for the reference item was determined after 24 h. The EC100 value was determined directly from the test results. The EC10 and EC50 with 95 % confidence interval (CI) were determined by probit analysis according to standard procedures.
EC- values after 24 h of the reference item in mg/L were as follows:
EC10 : 1.2
EC50 : 1.9 (CI 1.7-2.1)
EC100 : 5.8
The ECso - value of reference item potassium dichromate after 24 h is within the prescribed concentration range of 1.0 - 2.5 mg/L of validity criteria according to AQS: DIN Guideline 38412 L 30.
- Other:
None. - Reported statistics and error estimates:
- An estimate of the EC50 value at 3 hours was given by inspection of the immobilisation data.
The EC50 value and associated confidence limits at 24 hours were calculated by the trimmed Spearman-Karber method (Hamilton et al 1977) and the EC50 value and associated confidence limits at 48 hours and the slope of the response curve and its standard error were calculated by the maximum-likelihood probit method (Finney 1971) using the ToxCalc computer software package (Toxcalc 1999).
Probit analysis is used when two or more partial responses to exposure are shown.
When only one partial response is shown the trimmed Spearman-Karber method is appropriate.
Any other information on results incl. tables
Table 1. Percentage number of daphnids incapable of swimming after 24 and 48 h of exposure (n = 20)
IMMOBILISATION [%] |
||||||||||
Limit Conc. [mgDOC/L] |
24 h |
48 h |
||||||||
Replicates |
Replicates |
|||||||||
1 |
2 |
3 |
4 |
MV |
1 |
2 |
3 |
4 |
MV |
|
0.13 Control |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
0 |
MV = Mean value
Applicant's summary and conclusion
- Validity criteria fulfilled:
- yes
- Conclusions:
- In the tested limit concentration (0.13 mg DOC/L) there is no biologically significant effect neither in the tested limit concentration nor in control group.
- Executive summary:
In the acute immobilisation test with Daphnia magna (STRAUS) the effect of the saturated solution as limit concentration (0.13 mg DOC / L) of the test item Lanolin alcohol (Wollwachsalkohol/Lanolinalkohol).
Batch number Eucerit 6480, was determined according to OECD Guideline 202 I/EEC Directive 92/69/EEC Method C.2. The limit test was conducted from 12 -06 -2001 to 14 -06 -2001 at DR.U.NOACK-LABORATORIUM FOR ANGEWANDTE BIOLOGIE D-31157 Sarstedt.
The test item was clearly dissolved at the limit concentration.
The study was conducted under static conditions over a duration of 48 hours. 20 test organisms were exposed to the limit test concentration and control.
Table 1. Percentage number of daphnids incapable of swimming after 24 and 48 h of exposure (n = 20)
IMMOBILISATION [%]
Limit
Conc.
[mgDOC/L]
24 h
48 h
Replicates
Replicates
1
2
3
4
MV
1
2
3
4
MV
0.13
Control
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
0
MV = Mean value
A reference test is carried out once per month with potassium dichromate as reference item and the reference toxicity determined. The ECso -value of the reference item at 1.9 mg/l after 24 h was within the prescribed concentration range of 1.0 to 2.5 mg/l according to AQS, DIN Guideline 38412 l 30.
Water quality parameters of pH-value and dissolved oxygen concentration measured at 0 and 48 h were determined to be within the acceptable limits.
The validity criteria of the test guideline were fulfilled.
In the tested limit concentration (0.13 mg DOC/L) no biologically significant effect was determined.
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