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Diss Factsheets

Environmental fate & pathways

Bioaccumulation: aquatic / sediment

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Administrative data

Link to relevant study record(s)

bioaccumulation in aquatic species: fish
Type of information:
experimental study
Adequacy of study:
weight of evidence
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
The study was well documented and meets generally accepted scientific principles, but was not conducted in compliance with GLP.
Principles of method if other than guideline:
Flow-through study with radiolabelled test substance
GLP compliance:
Details on preparation of test solutions, spiked fish food or sediment:
STOCK AND TEST SOLUTION AND THEIR PREPARATION:  Super stock solutions were prepared by diluting known  amounts of labelled and unlabelled test substance with  distilled water. Diluter stock solutions were prepared  by further dilution with distilled water. Test solutions  were prepared using an intermittent-flow proprtional diluter. The specific activities of the super stock solutions were  determined to be: 0.14 mg/l stock: 335 ± 24 disintegrations per minute/µg 1.4 mg/l stock solution: 33.2 ± 2.5 dpm/µg
Test organisms (species):
Cyprinus carpio
Details on test organisms:
TEST ORGANISMS: Size: 19 ± 2 cm Weight: 39 ± 3 g Pretreatment: acclimated for 30 days before testing. Cumulative mortality less than 2% during holding period Feeding: Dry pelleted ration twice daily ad libitum
Route of exposure:
Test type:
Water / sediment media type:
natural water: freshwater
Total exposure / uptake duration:
49 d
Total depuration duration:
14 d
35 mg/l as CaCO3
Test temperature:
18 ± 1.0 °C
Dissolved oxygen:
> 6.0 mg/l (60% saturation)
Nominal and measured concentrations:
Nominal: 0.14 mg/l and 1.4 mg/l
Mean measured: 0.14 mg/l and 1.2 mg/l
Key result
Conc. / dose:
0.14 mg/L
22 L/kg
non-edible fraction


A mean fish concentration of 3.1 mg/kg, equivalent to BCF = 22, was  determined for non-edible tissues, although no meaningful trends in  bioaccumulation were observed at 7 sampling points over the exposure  period. Analysis of the gut, faeces and visceral  tissues at Day 42 showed  that 72% of residues were present in faecal matter, 16% in the gut and  12% in the remaining visceral tissues, indicating that the substance was  ingested and eliminated with very little accumulation.  Expressed on a  whole-body basis, the mean fish concentration was < 0.6 mg/kg, equivalent  to BCF < 4.


A similar pattern to the lower test concentration was The mean fish concentration determined for non-edible tissues was  12.2 mg/kg, equivalent to BCF = 10.  Analysis on Day 42 showed 45% in  faecal matter, < 5% in the gut and 50% in the remaining 

visceral tissues.  Accumulation in edible tissues was not observed.  

ELIMINATION: Elimination of the test substance from non-edible tissues was rapid and  extensive. After the 14 day depuration, 96% of accumulated substance was  eliminated at the 0.14 mg/l nominal test concentration; 93% was  eliminated at the 1.4 mg/l nominal test concentration.

BCF values of BCF <4 - 22 were determined in a reliable study conducted according to generally accepted scientific principles.

Description of key information

No study of bioaccumulation conducted with ATMP-N-oxide 5K is available. Given the very low estimated value of log Kow (-4.3), bioaccumulation is expected to be very low. Two weight of evidence studies are available from the read-across substance, ATMP-H which indicate a bioconcentration factor in the exposure phase of <4 – 22 and 5.2 – 17.7.

Key value for chemical safety assessment

BCF (aquatic species):
22 L/kg ww

Additional information

No data are available on bioaccumulation of ATMP-N-oxide-5K. However, a study on bioaccumulation of ATMP-H (CAS 6419-19-8) in carp, bioconcentration factor in the exposure phase of –<4 - 22 (EG&G, 1976). A secondary source reports results of a separate test as BCF = 5.2 – 17.7 measured for ATMP acid in Brachydanio rerio (zebra fish) (Steber and Wierich). A third study reporting consistent results, previously reported in the public domain via the SIDS dossier, is not traceable.

The key result is read across to this substance from ATMP-H.