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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Short-term toxicity to fish:

The acute toxicity of Guanidine Nitrate to fish was tested in toxicity tests against Ictalurus punctatus (Channel catfish), Pimephales promelas (Fathead minnow) peer-reviewed by van der Schalie (1985) and Leuciscus idus (Goldorfe) reported by Adema (1988). The lowest 96-h-LC50 was reported as 690 mg a.i./L in the toxicity test against Pimerphales promelas.


Long-term toxicity to fish:

The chronic toxicity of Guanidine Nitrate was tested in a 35-day fish early life stage test (FELS) against Fathead minnow (van der Schalie, 1985). The NOEC was 181mg/L and the lowest effect concentration was reported at 424mg/L (LOEC).


Short-term toxicity to aquatic invertebrates:

The 48hr-acute toxicity of Guanidine Nitrate to aquatic invertebrates was studied. The immobilization of Daphnids was used as the critical criteria. The study of the acute toxicity of Guanidine Nitrate against Daphnia magna in the report of van der Schalie (1985) shows that the 48-h-EC50, the concentration, in which 50 per cent reduction in immobilization occurs, is 70.2mg/L.


Long-term toxicity to aquatic invertebrates:

The long-term toxicity of Guanidine Nitrate to Daphnia magna was tested in a 21-day flow-through test. The lowest concentration with an effect on reproduction (LOEC) was monitored at a concentration 4.2mg/L in a first chronic test of van der Schalie, 1985 and 6.09mg/L in a second chronic test. The highest concentration of the test item without an effect (NOEC) was found at 2.90mg/L. In another study of Cooney et al. (1985) the 21-day-LOEC was higher (9.95mg/L). It is interesting to mention that Guaninine Nitrate is 10 to 20 times more toxic in a chronic 21-day toxicity test compared to an acute 48h toxicity test which is reported in the same study of van der Schalie, 1985.


Toxicity to aquatic algae and cyanobacteria: 

In a 72 hour acute toxicity study (Seibersdorf, 2010), the cultures of Pseudokirchneriella subcapita were exposed to Guanidine Nitrate. The 72h-EC50 values based on yield ( and growth rate) were 6.3mg a.i./L (6.3 mg a.i./L) and the 72h-NOEC values based on yield (and growth rate) were 11.8 mg a.i./L (33.5 mg a.i./L), respectively.


Toxicity to microorganisms: 

The toxicity of Guanidine Nitrate to microorganisms was studied in a 18-h-cell multiplication inhibition test using Pseudomonas putida as test organism (SKW, performed by NOTOX, 1988). Bacterial growth inhibtion was determined by measurement of the turbidity of the bacterial culture.

A 18-h-EC10 of 831.8mg/L was determined.

Additional information