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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Basic toxicokinetics

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Administrative data

Endpoint:
basic toxicokinetics in vitro / ex vivo
Type of information:
experimental study
Adequacy of study:
key study
Study period:
2012
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well-documented study report which meets basic scientific principles

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2013

Materials and methods

Test guideline
Qualifier:
no guideline available
Principles of method if other than guideline:
Determination of in vitro hydrolysis rates of methacrylate esters; determination of half-lifes in rat liver microsomes and whole rat blood.
GLP compliance:
yes

Test material

Constituent 1
Chemical structure
Reference substance name:
2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate
EC Number:
254-588-0
EC Name:
2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate
Cas Number:
39670-09-2
Molecular formula:
C12H22O5
IUPAC Name:
2-[2-(2-ethoxyethoxy)ethoxy]ethyl methacrylate
Constituent 2
Reference substance name:
2-(2-(2-ethoxy ethoxy)-ethoxyethyl methacrylate
IUPAC Name:
2-(2-(2-ethoxy ethoxy)-ethoxyethyl methacrylate
Test material form:
other: liquid
Details on test material:
- Name of test material (as cited in study report): ethoxytriethylene glycol methacrylate
Radiolabelling:
no

Test animals

Species:
other: rat liver microsomes and rat blood

Administration / exposure

Vehicle:
DMSO
Duration and frequency of treatment / exposure:
120 min (samples collected at 0, 2, 5, 15, 30, 60 and 120 minutes)
Doses / concentrations
Remarks:
Doses / Concentrations:
0.25 mM
No. of animals per sex per dose / concentration:
not applicable; in vitro test
Control animals:
other: not applicable; in vitro test
Positive control reference chemical:
Methyl methacrylate
Details on dosing and sampling:
METABOLITE CHARACTERISATION STUDIES
- Method type(s) for identification: liquid chromatography separation with accurate mass quadrupole/time-of-flight mass spectrometry detection (LC/QTOF-MS) to quantitate methacrylic acid concentrations
- Limits of detection and quantification: LLQ = 0.0117 mM methacrylic acid

Results and discussion

Main ADME results
Type:
metabolism
Results:
the ester was rapidly converted to MAA in whole rat blood and rat liver microsomes: half life 4.95 min (liver microsomes) / 27.72 min (blood)

Any other information on results incl. tables

Negative controls in the rat liver microsome experiments included incubations with heat-inactivated microsomes, no microsomes and no NADPH. Removal of NADPH made little or no difference in hydrolysis rates. Heat inactivation significantly reduced hydrolysis rates, and absence of microsomes resulted in no hydrolysis. 

ET3EGMA was rapidly converted to MAA in whole rat blood and rat liver microsomes with hydrolysis half-lives of 4.95 min (liver microsomes) and 27.72 min (blood).

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): other: The metabolism data show that ET3EGMA is rapidly hydrolysed in vitro.
The metabolism data show that ET3EGMA is rapidly hydrolysed in vitro.
Executive summary:

This in vitro metabolism study was conducted to investigate in vitro hydrolysis rates of ET3EGMA. Half-lifes were determined in rat liver microsomes and whole rat blood.

ET3EGMA was rapidly converted to MAA in whole rat blood and rat liver microsomes with hydrolysis half lives of 4.95 min (liver microsomes) and 27.72 min (whole blood)