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Ecotoxicological information

Short-term toxicity to fish

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Description of key information

The geometric mean of LC0 and LC100 for dichloromethylbenzene mixture to Danio rerio was determined to be 2.2 mg/L for 96h. The LC0 was 1.8 mg/L and the LC100 was 2.6 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Effect concentration:
2.2 mg/L

Additional information

Key study

In a 96-h acute toxicity study, Danio rerio were exposed to the dichloromethylbenzene mixture at nominal concentrations of 2.8 - 44 mg/L under semi-static conditions. Analytical monitoring was performed. The authors determined the 96-h LC0 to be 2.2 mg/L and the LC100 to be 4.3 mg/L. The LC0 and LC50 values were recalculated during dossier preparation as geometric mean values based on the measured concentrations, resulting in an LC0 of 1.8 mg/L and an LC100 of 2.6 mg/L. The geometric mean of these endpoints was determined to be 2.2 mg/L.

The study was performed according to GLP and followed a method which is comparable with the required guideline and is therefore considered to fulfil the requirements for a key study.

Reference: Caspers & Müller, 1992

Supporting studies

In a 96-h acute toxicity study, Oryzias latipes were exposed to 2,4-dichlorotoluene at nominal concentrations of 1.0 - 10 mg/L under semi-static conditions. The OECD-Guideline 203 was followed. Analytical monitoring was not performed.

A 96-h LC50 of 2.7 mg/L was determined based on nominal concentrations. A vehicle was used and controls with and without vehicle were run.

Reference: EA Japan, 1992

In a 96-h acute toxicity study, Pimephales promelas were exposed to 3,4-dichlorotoluene. It was a flow-through system and the tested concentrations were between 0.85 and 6.59 mg/L (nominal) and between 0.7 and 5.35 mg/L (mean measured).

A 96-h LC50 of 2.91 mg/L was determined based on mean measured concentrations.

Reference: Brooker, 1984

In a 96-h acute toxicity study, Danio rerio were exposed to 2,3-dichlorotoluene. It was a static system. Due to a very steep dose-response-curve, an LC50 could not be estimated. A 96-h LC0 of 42.2 mg/L and an LC100 of 56.2 mg/L were determined based on nominal concentrations.

The geometric mean of these values was calculated to be 48.7 mg/L.

Reference: Bernhofen-Klinke, 1985

In a 96-h acute toxicity study, Oryzias latipes were exposed to 2,6-dichlorotoluene.

A 96-h LC50 of 6.4 mg/L was determined.

Reference: Ueda, 1993

In a 96-h acute toxicity study, Danio rerio were exposed to an isomeric mixture with a higher amount of 2,6-dichlorotoluene. It was a flow-through test system and analytical monitoring was performed.

A 96-h LC50 of 3.24 mg/L was determined.

Reference: FhG, 1989

Further study

In a 96-h acute toxicity study, Danio rerio were exposed to 2,4-dichlorotoluene. Further details are not available.

A 96-h LC0 of > 100 mg/L was determined based on nominal concentrations.

Since only a short abstract is available and the determined endpoint is not relevant, the study is considered not to be reliable.

Reference: Bayer, <= 1995