Disodium [2,4-dihydro-4-[(2-hydroxy-5-nitrophenyl)azo]-5-methyl-2-phenyl-3H-pyrazol-3-onato(2-)][3-hydroxy-4-[(2-hydroxy-1-naphthyl)azo]-7-nitronaphthalene-1-sulphonato(3-)]chromate(2-)

Endpoint summary

Additional information

Adequate chronic toxicity data are not available for Acid Brown 282 (ABr282), therefore the aquatic toxicity assessment and classification are based mainly on the short-term toxicity data available.

Despite ABr282 has a log kow lower than 4 (indicating a low potential for bioaccumulation), it is not rapidly degradable.

According to the CLP Regulation (EC 1272/2008), acute aquatic toxicity is normally determined using a fish 96-hour LC50 , a crustacean species 48-hour EC50 and/or an algal species 72- or 96-hour EC50; nevertheless it is also specified in the Regulation that data on other species (e.g. Lemna spp.) shall also be considered if the test methodology is suitable. These species cover a range of trophic levels and taxa and are considered as surrogate for all aquatic organisms.

Data are available for fish (different specie), Daphnia magna and Lemna minor.

According to the CLP Regulation (EC 12720/2008) criteria for the identification of the classification categories for hazardous to the aquatic environment, all studies about short-term toxicity to fish fixed the short-term effect level on fish between 1 and 10 mg/l; in the Lemna study the effects were recorded in the range of 10 - 100 mg/l.

The outcomes of the tests performed on the three trophic levels suggest that fish is the most sensitive species, thus the classification has to be based on the short-term toxicity to fish.

In conclusion, the ABr282 is classified as Aquatic Chronic 2 (H411) according to the CLP Regulation (EC 1272/2008).