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EC number: 268-626-9 | CAS number: 68131-73-7
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
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Toxicity to aquatic algae and cyanobacteria
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to aquatic algae and cyanobacteria
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 08-05-1990 to 12-05-1990
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: study is complete, was performed under GLP. however due to the absence of chemcal analysis, the use in risk assessments is restricted, especially when looking at bioavailability.
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 201 (Alga, Growth Inhibition Test)
- GLP compliance:
- yes
- Analytical monitoring:
- no
- Vehicle:
- no
- Details on test solutions:
- For the definitive test a stock solution of 0.01 g/litre was
prepared by dissolving the test substance in deionized water.
This stock solution was titrated to a neutral pH. The selected
test concentrations were prepared by dilution of this stock
solution in the inoculated test medium. Based on the results of
prelimanary experiments the
definitive test were selected. - Test organisms (species):
- Raphidocelis subcapitata (previous names: Pseudokirchneriella subcapitata, Selenastrum capricornutum)
- Details on test organisms:
- The test was carried out with the unicellular green algae;
Selenastrurn capricornutum ATCC 22662. This strain was maintained
on mineral salts agar slants in the light at room temperature. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 72 h
- Test temperature:
- 22.5-23 C
- pH:
- 6.9-7.7
- Nominal and measured concentrations:
- nominal: 0, 0.080, 0.160, 0.320, 0.640, 1.280 mg/L
- Details on test conditions:
- The culturing apparatus is a cabinet in which the temperature is
maintained between 22° and 24°C, and continuous uniform
illumination is provided in the spectral range of 400 to 700 nm.
The light intensity is in the range of 6000 to 10000 lux which
is obtained by using seven 30 W fluorescent lamps (colour
temperature of approximately 4300 K), at a distance of 0.35 m
from the algal culture. The culture flasks were rotated
continuously at 100 rpm to prevent sedimentation of the algae. - Reference substance (positive control):
- yes
- Remarks:
- pottassium dichromate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- growth rate
- Duration:
- 72 h
- Dose descriptor:
- EC50
- Effect conc.:
- 0.23 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- biomass
- Duration:
- 72 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.16 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- other: Both growth rate and biomass
- Details on results:
- The ErC50 and EbC50 (0-72 h) of higher ethylene polyamines are - 0.5 and
0.23 mg/litre respectively. The NOEC determined of higher ethylene polyamines is 0.16 mg/litre. - Results with reference substance (positive control):
- Are in line with guideline requirements
- Reported statistics and error estimates:
- The ECSO values were computed from the best fitted line (least
square method) through the points given by the probit of the
percentage inhibition and the logarithm of the concentration of
the test substance. The ECSO value calculated with the area
under the growth curve is termed EbC 50 (0-72 h) whereas the ECvalue calculated with the specific growth
rate is termed ErC50
Confidence limits were computed based on Fieller's theoreme All
computations were performed with the software package SAS
(release: 6.03). - Validity criteria fulfilled:
- yes
- Conclusions:
- The ErC50 and EbC50 (0-72 h) of PEPA are 0.5 and 0.23 mg/litre respectively. The NOEC determined of
PEPA is 0.16 mg/litre. The study was performed under GLP and meets all validity criteria. Only nominal test concentrations are presented which limits the use of this data when performing a riskassessment when looking at the bio-available fraction - Executive summary:
The toxicity of PEPA to exponentially growing S. capricornutum has been assessed over an exposure period of 72 hours. The algal inhibition test was performed according to the EEC/OECD (201) test guidelines One minor deviation from the protocol of the algal inhibition test was introduced.
- The KH2PO4 and NaCO3 concentrations in the test medium were increased to 160 and 100 mg/litre, respectively, to maintain a constant pH.
- Preliminary experiments to determine the concentration range are performed but not reported according to the guidelines.
In the definitive test the algae were exposed to the following nominal concentrations of 0.080, 0.160, 0.320, 0.640 and 1.280 mg/litre. The EbC50 (0 -72 h) and ErC50 of PEPA forS. capricornutumare 0.5 and 0.23 mg/litre, respectively. The NOEC determined of tetraethylenepentamine is 0.16 mg/litre. All test concentrations are based on nominal. All validity criteria are met and thus the study is valid.
However the usability of the presented data islimited due to the absence of actual measured concentrations and therefore no information on the bioavailable fraction.
Reference
Description of key information
OECD guideline 201, Pseudokirchneriella subcapitata: EC50 (72 h) of 0.5 mg/L and NOEC (72 h): 0.16 mg/L based on algal growth rate
Key value for chemical safety assessment
- EC50 for freshwater algae:
- 0.5 mg/L
- EC10 or NOEC for freshwater algae:
- 0.16 mg/L
Additional information
The acute toxicity of the substance to aquatic algae was tested in a study set up according to OECD guideline 201. The freshwater algae Pseudokirchneriella subcapitata was exposed to nominal substance concentrations of 0.080, 0.160, 0.320, 0.640 and 1.280 mg/L. Based on the algal growth rate an EC50 (72 h) of 0.5 mg/L (nominal) was determined. The NOEC (72 h) was 0.16 mg/L for algal growth and biomass respectively.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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