Amines, polyethylenepoly-

Short-term toxicity to aquatic invertebrates

Link to relevant study record(s)

Reference

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

25-04-1989 to 03-05-1989

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: study is complete, was performed under GLP. however due to the absence of chemcal analysis, the use in risk assessments is restricted, especially when looking at bioavailability.

Qualifier:

according to guideline

Guideline:

EU Method C.2 (Acute Toxicity for Daphnia)

Qualifier:

according to guideline

Guideline:

other: S.A.L.M. Kooyman. "Parametric analyses of mortality rates in bio-assays". Water Research 15(1981)107-119.

GLP compliance:

yes

Analytical monitoring:

no

Vehicle:

no

Details on test solutions:

A stock solution of 25 g/l was prepared by dissolving the test
substance in demineralized water containing concentrated HCl.
The presence of the test substance in the test medium caused a
change of the pH, which is outside the range that can be supported
by the test animal. Therefore, the pH was neutralized
both in the stock solution and in th~ dilution water to a value
between 7.0 and 7.3. The chosen test concentrations were prepared
by dilution of the stock solution.

Test organisms (species):

Daphnia magna

Details on test organisms:

The test organism was Daphnia magna; they were produced in the
Akzo Research Laboratories Arnhem, dept. CRL according to NEN
6503 and Standard Operating Procedures SOP CRL T2. They were
less than 24 hours old at the beginning of the test.

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

48 h

Hardness:

73.1 mg/L Ca; 20.4 mg/L Mg; 14.9 dH

Test temperature:

19.7 - 20.8 C

pH:

7.3-7.9

Dissolved oxygen:

8.8-9.2 mg/L

Nominal and measured concentrations:

nominal: 0.56, 1.0, 1.8, 3.2, 5.6, 10 mg/L

Details on test conditions:

The temperature was kept between 19.7 and 20.8°C, and the light
regime was 16 hours ambient light per day.
The duration of the test was 48 hours.
The test vessels were 400 ml glass beakers, containing 250 ml
of test medium. Each beaker contained S test animals.

Reference substance (positive control):

no

Duration:

48 h

Dose descriptor:

EC50

Effect conc.:

2.2 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Remarks on result:

other: 1.9-2.6 mg/L

Details on results:

No effect on mobility was observed at 1.0 mg/l and 100% immobilisation was observed
at 3.2 mg/l. The EC50-48 h is 2.2 mg/l. The 95% confidence
limits are 1.9 and 2.6 mg/l.

Reported statistics and error estimates:

The EC50 was determined by an LCSO program of Griffioen (RlZA)
based on a model of Kooyman (1981).

Nominal concentration mg/L	Total number of mobile animals
	0h	24h	48h
Control	20	20 (7)	20 (5)
0.56	20	20	20
1.0	20	20	20
1.8	20	20 (1)	17 (1)
3.2	20	20 (2)	0
5.6	20	16	0
10	20	15 (3)	0

() = swims but sticks at the surface

Validity criteria fulfilled:

yes

Conclusions:

the EC50 was determined to be 2.2 mg/L after 48h exposure to HEPA of Daphnia magna. The study is valid. No analysis has been performed so actual concentrations are unknown, this makes the reliability restricted when using the value for riskassessment purposes.

Executive summary:

The fraction of HEPA (Higher ethylene polyamines) was tested in an acute toxicity test with the water flea (Daphnia magna). The test was performed according to the EEC method, part C.: Methods for the determination of ecotoxicity, C.2. Acute toxicity for Daphnia.

The test was carried out as a static test, in which the solution was not renewed.

No mortality was observed up to 1.0 mg/l. The EC50 was 2.2 mg/l, with 95% confidence limits of 1.9 and 2.6 mg/l. All test concentrations were nominal, this limits the use of the endpoint for risk assessment purposes.

Description of key information

 EU method C.2 , Daphnia magna: EC50(48 h) of 2.2 mg/L (nominal) based on mobility 

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates

Effect concentration:

2.2 mg/L

Additional information

The short-term toxicity of the substance was tested according to EU method C.2. The study was conducted with Daphnia magna as test organisms. The daphnids were exposed to nominal substance concentrations of 0.56, 1.0, 1.8, 3.2, 5.6 and 10 mg/L. The test was terminated after 48 hours. After 48 hours an effect on the mobility was not observed at a test substance concentration of 1 mg/L. Whereas 100% of the test organisms were immobile at a concentration of 3.2 mg/L at test termination. The determined EC50(48 h) is 2.2 mg/L (nominal).