disodium 4-amino-6-((4-((4-(2,4-diaminophenyl)azo)phenylsulfamoyl)phenyl)azo)-5-hydroxy-3-((4-nitrophenyl)azo)naphthalene-2,7-disulfonate

Toxicity to aquatic plants other than algae

Link to relevant study record(s)

Reference

Endpoint:

toxicity to aquatic plants other than algae

Type of information:

experimental study

Adequacy of study:

key study

Study period:

2012, December

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Qualifier:

according to guideline

Guideline:

OECD Guideline 221 (Lemna sp. Growth Inhibition Test)

Deviations:

yes

Remarks:

the frond number was counted at day 2

GLP compliance:

yes (incl. QA statement)

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material (migrated information):
water solubility: 150 g/l

Analytical monitoring:

no

Vehicle:

no

Test organisms (species):

Lemna minor

Details on test organisms:

Exponential growing plant monocolture of Lemna Minor (Umweltbundesamt, D-06813 Dessau-Rosslau, Germany

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

7 d

Test temperature:

25 ± 1 °C

pH:

5.5 ± 0.2

Nominal and measured concentrations:

The test were performed at concentrations of 20, 200 and 2000 mg/l of the active ingredient.
Assuming a purity of 65%, nominal concentrations of 30.8, 308 and 3080 mg/l, respectively, were prepared.

Details on test conditions:

TEST SYSTEM
- Test vessel: 400 ml beaker
- Material, size, headspace, fill volume: glass with 200 ml of test medium

GROWTH MEDIUM
- Standard medium used: yes
Test medium STEINBERG with the following composition:
Macroelements mg/l
KNO3 350
KH2PO4 90
K2HPO4*3H2O 16.5
MgSO4*7H2O 100
Ca(NO3)2*4H2O 295
Microelements μg/l
H3BO3 120.0
ZnSO4*7H2O 180.0
Na2MoO4*2H2O 44.0
MnCl2*4H2O 180.0
FeCl3*6H2O 760.0
C10H14N2Na2O8*2H2O 1500.0

ILLUMINATION: Continuos (3500 - 7500 lux) from Osram Fluora L18W77 (Osarm AG, Winterthur, Switzerland)

Reference substance (positive control):

no

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

> 2 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

growth rate

Duration:

7 d

Dose descriptor:

EC50

Effect conc.:

> 2 000 mg/L

Nominal / measured:

nominal

Conc. based on:

act. ingr.

Basis for effect:

biomass

Reported statistics and error estimates:

Dunnett's test using the ToxRat Standard Version 2.10

Validity criteria fulfilled:

yes

Conclusions:

Acid Black 210 tested for OECD 221 shows EC50, grow rate > 2000 mg/l and EC50, biomass between > 2000 mg/l for dry weight.

Executive summary:

The inhibitory effect based on the average specific growth rate and the yield (duckweed biomass production) of Acid Black 210 to the duckweed Lemna minor was investigated over a period of 7 d.

The test was performed at concentrations of 20, 200 and 2000 mg/l of the active ingredient. Assuming a purity of 65%, nominal concentrations of 30.8, 308 and 3080 mg/l, respectively, were prepared.

With respect to duckweed growth rate for the endpoint frond numbers, the following effects were observed: 3080 mg/l (30%), 308 mg/l (22%), 30.8 mg/l (10%).

Based on these data the median effect concentration (E_rC₅₀) with respect to growth rate was estimated to be >3080 mg/l.

With respect to duckweed yield for the endpoint frond numbers, the following effects were observed: 3080 mg/l (57%), 308 mg/l (46%) and 30.8 mg/l (24%).

The median effect concentration with respect to yield (E_yC₅₀) for the endpoint frond numbers of Acid Black 210 toLemna minorwas estimated to be 308 - 3080 mg/l nominal concentration.

The no-observed-effect concentration with respect to growth rate (NOE_rC) and yield (NOE_yC) for the endpoint frond numbers was <30.8 mg/l nominal concentration.

With respect to duckweed growth rate for the endpoint dry weight the following effects as compared to the untreated controls were observed at the respective nominal concentrations: 3080 mg/l (21%), 308 mg/l (21%), 30.8 mg/l (12%).

With respect to duckweed yield for the endpoint dry weight the following effects as compared to the untreated controls were observed at the respective nominal concentrations: 3080 mg/l (45%), 308 mg/l (45%) and30.8 mg/l (30%).

Based on these data the median effect concentration with respect to growth rate (E_rC₅₀)and yield (E_yC₅₀)for the endpoint dry weight of Acid Black 210 to Lemna minorwas estimated to be >3080 mg/l nominal concentration.

The no-observed-effect concentration with respect to growth rate (NOE_rC) and yield (NOE_yC) for the endpoint dry weight was <30.8 mg/l nominal concentration.

Description of key information

EC 50 > 2000 mg/l for dry weight

Key value for chemical safety assessment

EC50 for freshwater plants:

2 000 mg/L

Additional information

One study was performed (Alexa Caduff, 2012) following OECD 221 on Acid Black 210.

The tested aquatic plant is Lemna minor and the EC50 for grow rate and biomass is > 2000 mg/l.