Registration Dossier
Registration Dossier
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EC number: 205-483-3 | CAS number: 141-43-5
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in mammalian cells
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- test procedure in accordance with national standard methods with acceptable restrictions
- Remarks:
- No details on the concentrations used
Data source
Reference
- Reference Type:
- publication
- Title:
- Inhibition of Metabolic Cooperation in Chinese Hamster V79 Cells by Various Organic Solvents and Simple Compounds
- Author:
- Chen TH, et al.
- Year:
- 1 984
- Bibliographic source:
- Cell Biol. Toxicol. 1 (1):155- 171
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Method described in Trosko et al., 1984 (In Handbook of Carcinogen Testing) is based on metabolic cooperation between 6-thioguanine sensitive (HGPRT+) cells and 6-thioguanine resistant (HGPRT-) cells in vitro.
- GLP compliance:
- no
- Type of assay:
- in vitro mammalian cell gene mutation test using the Hprt and xprt genes
Test material
- Reference substance name:
- 2-aminoethanol
- EC Number:
- 205-483-3
- EC Name:
- 2-aminoethanol
- Cas Number:
- 141-43-5
- Molecular formula:
- C2H7NO
- IUPAC Name:
- 2-aminoethanol
- Test material form:
- liquid
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- Chinese hamster lung fibroblasts (V79)
- Details on mammalian cell type (if applicable):
- - Type and identity of media: Modified Eagle's medium
- Properly maintained: yes
- Periodically checked for Mycoplasma contamination: yes
- Periodically checked for karyotype stability: no data
- Periodically "cleansed" against high spontaneous background: no data - Additional strain / cell type characteristics:
- other: with and without HGPRT deficiency
- Metabolic activation:
- without
- Test concentrations with justification for top dose:
- not indicated
Controls
- Untreated negative controls:
- yes
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 12-tetra-decanoyl-1-phorbol-13-acetate (TPA)
Results and discussion
Test results
- Key result
- Species / strain:
- Chinese hamster lung fibroblasts (V79)
- Metabolic activation:
- without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not specified
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- valid
- Positive controls validity:
- valid
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
