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Diss Factsheets

Toxicological information

Eye irritation

Currently viewing:

Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
comparable to guideline study with acceptable restrictions

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1966
Report date:
1966

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
Principles of method if other than guideline:
BASF-Test
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
2-aminoethanol
EC Number:
205-483-3
EC Name:
2-aminoethanol
Cas Number:
141-43-5
Molecular formula:
C2H7NO
IUPAC Name:
2-aminoethanol
Test material form:
liquid

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White
Details on test animals or tissues and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.65-2.89 kg
- Diet: ad libitum
- Water: ad libitum

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: adjacent eye (physiological NaCl-solution)
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 0.05 mL
Duration of treatment / exposure:
not applicable (tests substance was not washed out)
Observation period (in vivo):
8 days
Number of animals or in vitro replicates:
2
Details on study design:
REMOVAL OF TEST SUBSTANCE
- Washing: none

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Irritation parameter:
cornea opacity score
Basis:
animal #2
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Irritation parameter:
cornea opacity score
Basis:
animal #3
Time point:
24/48/72 h
Score:
3
Max. score:
4
Reversibility:
not reversible
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
animal #1
Time point:
24/48/72 h
Score:
2
Max. score:
3
Reversibility:
not reversible
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
animal #2
Time point:
24/48/72 h
Score:
0.66
Max. score:
3
Reversibility:
fully reversible
Irritation parameter:
conjunctivae score
Remarks:
redness
Basis:
animal #3
Time point:
24/48/72 h
Score:
2
Max. score:
3
Reversibility:
fully reversible
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
24/48/72 h
Score:
0.66
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
24/48/72 h
Score:
1.33
Max. score:
4
Irritation parameter:
chemosis score
Basis:
animal #3
Time point:
24/48/72 h
Score:
2
Max. score:
4
Reversibility:
fully reversible
Irritation parameter:
iris score
Basis:
animal #1
Time point:
24/48/72 h
Score:
1.3
Max. score:
3
Irritation parameter:
iris score
Basis:
animal #2
Time point:
24/48/72 h
Score:
0
Max. score:
3
Irritation parameter:
iris score
Basis:
animal #3
Time point:
24/48/72 h
Score:
1.3
Max. score:
3
Irritant / corrosive response data:
Chemical burnings, scars on upper eyelid, purulent secretion, bloody discharge, conjunctival bleedings, iritis, staphyloma, ciliar injections.

Any other information on results incl. tables

 

Readings

Animal

Cornea

Iris

Conjunctiva

Additional findings

op

ar

red

ch

di

1 h

1

1

-

0

0

0

-

cornea black-red coloured, bloody secretion, constricted pupil, strong secretion

2

3

-

0

0

0

-

cornea black-grey coloured, bloody secretion, constricted pupil, strong secretion

3

3

-

0

0

0

-

cornea brown-red coloured, chemical burnings, brown-red secretion

24 h

1

3

-

0

2

1

-

chemical burnings

2

3

-

0

1

2

 

chemical burnings

3

3

-

0

0

2

-

cornea brown-red coloured, chemical burnings, brown-red secretion

48 h

1

3

-

2

2

1

-

crust formation (eyelids)

2

3

-

-

1

2

-

chemical burning of the eyeball and eyelid

3

3

-

2

0

2

-

purulent secretion

72 h

1

3

-

2

2

0

-

purulent secretion

2

3

-

0

0

0

-

purulent secretion, conjunctiva covered with purulent fur

3

3

-

2

0

2

-

purulent secretion,

8 d

1

3

-

2

2

0

-

purulent secretion

2

3

-

0

0

0

-

loss of hair

3

3

-

2

0

0

-

Staphyloma, ciliar injections, conjunctiva white and chemically burned, scar on upper eyelid

Mean

24 -72 h

1

3

-

1.33

2

0.66

-

 

2

3

-

0

0.66

1.33

-

 

3

3

-

1.33

0

2

-

 

Mean

24-72 h

1-3

3

-

0.88

0.89

1.33

-

 

- endpoint not addressed

Applicant's summary and conclusion

Interpretation of results:
Category 1 (irreversible effects on the eye) based on GHS criteria