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Ecotoxicological information

Short-term toxicity to fish

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Reference
Endpoint:
short-term toxicity to fish
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1989-09-25 to 1989-12-16
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Remarks:
No analysis was performed.
Qualifier:
according to guideline
Guideline:
EU Method C.1 (Acute Toxicity for Fish)
Version / remarks:
1984
Deviations:
yes
Remarks:
No analysis is performed.
Qualifier:
according to guideline
Guideline:
OECD Guideline 203 (Fish, Acute Toxicity Test)
Version / remarks:
1984
Deviations:
yes
Remarks:
No analysis is performed.
Principles of method if other than guideline:
No analysis was performed.
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
yes
Details on test solutions:
The test substance was immiscible with water at the test concentrations and therefore acetone was used as an organic solvent in the preliminary and the definitive test. In the definitive test a stock solution containing 320 g of test substance per acetone was used.
Test organisms (species):
Poecilia reticulata
Details on test organisms:
TEST ORGANISM
- Common name: Poecilia reticulata (guppy)
- Source: well-known local aquarium retailer
- Length at study initiation: approximately 2 cm
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
hardness of 23°dH
Test temperature:
25 ° and 26 °C
pH:
pH (0 h; fresh) = 8.2 - 8.3
pH (96 h; old) = 8.0 - 8.3
Dissolved oxygen:
O2 (48 h; old) = 6.4 - 6.8 mg/L
O2 (96 h; old) = 7.1 - 8.3 mg/L
Nominal and measured concentrations:
nominal concentrations: 10, 18, 32, 56, 100 mg test substance/L
Details on test conditions:
TEST SYSTEM
- Test vessel: As test vessels were used 2-1 covered glass aquaria which contained 1 L medium. the loading of the test vessels was approximately 0.6 g biomass/L.

TEST MEDIUM / WATER PARAMETERS
The diluting water was a synthetic water, Dutch Standard Water, according to NPR 6507 (1980, having a pH of 8.2 and a hardness of 13°dH. The temperature was kept between 25° and 26°C and light regime was 12 hours of ambient light per day.
Reference substance (positive control):
no
Key result
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
44.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Remarks on result:
other: 36.9 - 53.0 mg/L
Key result
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
18 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality (fish)
Details on results:
The no observed effect concentration (NOEC) after 96 h amounted to 18 mg/L, whereas 100 mg/L produced 100 % mortality within 24 hours.
No other effects such as deviations in behaviour or appearance of the fish were observed.
Results with reference substance (positive control):
No positive control
Reported statistics and error estimates:
The LC50 was determined with an LC50-programme of Griffioen (RIZA), based on a model of Kooyman (1981).
Sublethal observations / clinical signs:

Mortality in definitive test:










































































 



 Number of surviving animals



mg test substance/L



0 h



24 h



48 h



72 h



96 h



Control I



10



10



10



10



10



Control II (acetone)



10



10



10



10



10



10



10



10



10



10



10



18



10



10



10



10



10



32



10



10



10



10



9



56



10



4



3



3



2



100



10



0



-



-



-


Validity criteria fulfilled:
yes
Conclusions:
The LC50 (96 h) was calculated to be 44.2 mg/L with confidence limits of 36.9 and 53.0 mg/L. The no observed effect concentration (NOEC) after 96 hours amounted to 18 mg/L, whereas 100 mg/L produced 100 % mortality within 24 hours.
Executive summary:

Methyl-ethylketone peroxide in DMP was tested in an acute toxicity test with Poecilia reticulata (guppy) under semi-statci conditions in accordance with EEC method C.1 (1984) and OECD test Guideline 203 (1985).

Due to the immiscibility of the test substance with water the test concentration were prepared using acetone as a solvent. Each test concentration contained 100 uL acetone/L, as did one control.

The LC50 (96 h) was calculated to be 44.2 mg/L with confidence limits of 36.9 and 53.0 mg/L. The no observed effect concentration (NOEC) after 96 hours was 18 mg/L, whereas 100 mg/L produced 100 % mortality within 24 hours.

No other effects, such as deviation in the behaviour or appearance of fish were observed.

Description of key information

Methyl-ethylketone peroxide in DMP was tested for acute toxicity to fish to EU-method C.1 (1984) and OECD guideline 203 (1984). In this 96-hour semi-static toxicity test with Poecilia reticulata (guppy), the LC50 value was 44.2 mg/L.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
LC50
Effect concentration:
44.2 mg/L

Additional information

Methyl-ethylketone peroxide in DMP was tested for acute toxicity to fish to EU-method C.1 (1984) and OECD guideline 203 (1984). In this 96-hour semi-static toxicity test with Poecilia reticulata (guppy), the LC50 value was 44.2 mg/L. The no observed effect concentration (NOEC) after 96 hours was 18 mg/L, whereas 100 mg/L produced 100 % mortality.