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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Description of key information

The acute toxicity of the test item to the earthworm Eisenia foetida in an artificial soil test has been investigated and gave a 14-day LC50 value of greater than 1,000 mg/kg.  Correspondingly the No Observed Effect Concentration was 1,000 mg/kg.

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
1 000 mg/kg soil dw

Additional information

A key study was performed in accordance with OECD Guideline No. 207 to assess the acute toxicity of the test item to the earthworm (Eisenia foetida) in an artificial soil. 

Following a preliminary range-finding test, 60 earthworms (six replicates of 10 worms) were exposed to a single concentration of 1,000 mg/kg of soil for a period of 14 days at a temperature of 20 to 23 degrees C. The number of mortalities were determined after 7 and 14 days exposure. A positive control using chloroacetamide, conducted approximately every 6 months, was reported for reference purposes.

The 14-day LC50 based on nominal test concentrations was greater than 1,000 mg/kg. The No Observed Effect Concentration was 1,000 mg/kg. The result of the positive control gave a 14-day LC50 for chloroacetamide of 32 mg/kg with 95% confidence limits of 29 to 35 mg/kg. The No Observed Effect Concentration was 18 mg/kg.