Registration Dossier
Registration Dossier
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EC number: 231-765-0 | CAS number: 7722-84-1
Toxicity to other aquatic organisms
Administrative data
- Endpoint:
- toxicity to other aquatic vertebrates
- Type of information:
- experimental study
- Adequacy of study:
- supporting study
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Range finding study - documentation insufficient for assessment.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- other: MicroBioTests Inc. SOP ROTOXKIT M
- GLP compliance:
- no
Test material
- Reference substance name:
- Hydrogen peroxide
- EC Number:
- 231-765-0
- EC Name:
- Hydrogen peroxide
- Cas Number:
- 7722-84-1
- Molecular formula:
- H2O2
- IUPAC Name:
- hydrogen peroxide
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test organisms
- Test organisms (species):
- other: Branchionus plicatilis
- Details on test organisms:
- Test organism: Branchionus plicatilis
Test organism source: MicroBioTest Inc., Belgium
Batch Number BP-200
Test organism life stage: Newly hatched (<2 h)
Study design
- Total exposure duration:
- 24 h
Test conditions
- Test temperature:
- 25 ± 2 °C
- Nominal and measured concentrations:
- nominal: first test: 0, 1.76, 3.52, 7.04, 14.08, 70.40 mg/L H2O2
second test: 0, 2.6, 3.5, 4.7, 6.2, 8.3 mg/L H2O2 - Details on test conditions:
- This test is commercially available as 'Testkit' at MicroBioTest Inc., Belgium, under the name ROTOXKIT M and performed according to the Standard Operational Procedure provided by the manufacture.
Test chamber: Microwell plate
Test solution volume: 0.3 mL
Number of organisms/chamber: 5
Replicates: 6
Dilution water: Standard seawater provided with kit
Photoperiod: Permanent dark
Test protocol: MicroBioTests Inc. SOP ROTOXKIT M - Reference substance (positive control):
- yes
- Remarks:
- Potassium dichromate (K2Cr2O7)
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 4.8 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: first test, 95% confidence interval 4.2-5.6 mg/L
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: first test
- Duration:
- 24 h
- Dose descriptor:
- EC50
- Effect conc.:
- 5.9 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: second confined test, 95% confidence interval of 5.7-6.2 mg/L
- Duration:
- 24 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.5 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: second confined test
- Details on results:
- An EC50 value of 4.8 mg/L H2O2 with a 95% confidence range of 4.2-5.6 mg/L was determined for the rotifer. The NOEC was 3.5 mg/ H2O2. Based on the results of the first test, a more confined concentration series was applied. The second test revealed an EC50 value of 5.9 mg/L with a 95% confidence interval of 5.7-6.2 mg/L. The NOEC was measured at 3.5 mg/L.
Applicant's summary and conclusion
- Conclusions:
- The second test revealed an EC50 value of 5.9 mg/L with a 95% confidence interval of 5.7-6.2 mg/L. The NOEC was measured at 3.5 mg/L.
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