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Long-term toxicity to aquatic invertebrates

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In a key semi-static 21-day long-term Daphnia magna test (OECD 211; KS=1) using the WAF of Distillate Aromatic Extract (CAS 64742-05-8), ten replicate test chambers (each containing one daphnid) were prepared for each treatment group and control. Two actual loadings were tested, namely 0.1 and 1.1 mg/l. The 21 day NOELR for reproduction was 0.1 mg/l, the LOELR was 1.1 mg/l (ExxonMobil Biomedical Sciences (2012).

In a supporting semi-static 21 -day long-term Daphnia magna reproductive test (OECD 211; KS=1), 40 animals/loading were exposed to the water accommodated fraction of distillate aromatic extract at nominal concentrations of 0, 10, and 1000 mg/L. Concentrations of the test substance were not determined during the study. The 21 day EL50 was >1000 mg/L and the NOEL was 1000 mg/L based on immobilisation and reproduction, respectively (BP Oil Europe, 1995).