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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Environmental fate & pathways

Additional information on environmental fate and behaviour

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Administrative data

additional information on environmental fate and behaviour
Type of information:
experimental study
Adequacy of study:
supporting study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference Type:
Population dynamics of free-floating and attached bacteria in a styrene-degrading biotrickling filter analyzed by denaturing gradient gel electrophoresis
Tresse, O. et al.
Bibliographic source:
Appl. Microbiol. Biotechnol. 59: 585-590

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
PCR-amplified 16S rDNA fragments from planktonic and sessile cells within a biotrickling filter were analyzed using denaturing gradient gel electrophoresis (DGGE), a phylogenic fingerprinting technique.
GLP compliance:
Type of study / information:
Microbiological population dynamics in a biotrickling filter after long-term exposure to high concentrations of styrene

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:

Results and discussion

Any other information on results incl. tables

The complexity of the sessile cells was always more pronounced than the complexity of the planktonic cells. The difference between the free-floating and attached bacteria suggests that two distinct communities were living in the BTF.

Throughout the 182 -day operating time, one band remained specific to the biofilm, which was related Rhodococcus erythropolis (100% homology in sequencing analysis), a widely distributed organic-pollutant degrader with hyphae.

Only 50% of the bands representing the inoculum were present in the established biomass, suggesting that the biomass established in the BTF was subjected to further enrichment compared to the inoculum culture, even though the inoculum was adapted to styrene.

The spatio-temporal analysis revealed a stability of the biofilm microbial complexity after an acclimation period (of 35 days).

Applicant's summary and conclusion