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Toxicity to microorganisms

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The toxicity of mineral oil contaminated soil samples during a 93-day bioremediation period was tested using the Microtox ™ test, which measures the reduction of light production byVibrio fischeri. The nominal concentration tested was 2000 mg/dry weight soil. The initial IC50 following application of mineral oil was 100% of the reference dose. After 93 days of mineral oil application to the soil, the Microtox TM test showed inhibition of 50 to 75% of the reference dose. The chronic LOEL was <2000 mg mineral oil/dry weight soil (Lee et al. 2002).

Supporting data estimated from the PETROTOX computer model show no acute toxicity to microorganisms at or below its maximum attainable water solubility (Redman et al., 2010b).