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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Meets generally accepted scientific standards, well documented and acceptable for assessment

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1972
Report date:
1973

Materials and methods

Principles of method if other than guideline:
50 µl of the test substance was applied to the conjunctival sac of 2 white Vienna rabbits and not rinsed off. The animals were 1 and 3 h after instillation and on day 1,2, 3, 4 and 8. Eye reactions were recorded and graded similar to the Draize scheme of the OECD test guideline 405.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Aniline
EC Number:
200-539-3
EC Name:
Aniline
Cas Number:
62-53-3
Molecular formula:
C6H7N
IUPAC Name:
aniline
Details on test material:
- Name of test material (as cited in study report): aniline
- Analytical purity: no data

Test animals / tissue source

Species:
rabbit
Strain:
Vienna White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
not required
Amount / concentration applied:
TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 50 µL; undiluted
Duration of treatment / exposure:
1 h - 8 days
Observation period (in vivo):
1 h - up to 8 days
Number of animals or in vitro replicates:
2
Details on study design:
The test substance remained in the eye.

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
animal #1
Remarks:
mean
Time point:
other: 24 - 72 h
Score:
2.3
Reversibility:
not reversible
Remarks on result:
other: observation period: 8 days
Irritation parameter:
cornea opacity score
Basis:
animal #2
Remarks:
mean
Time point:
other: 24 - 72 h
Score:
2
Reversibility:
not reversible
Remarks on result:
other: observation period: 8 days
Irritation parameter:
conjunctivae score
Basis:
animal #1
Time point:
other: 24 - 72 h
Score:
1
Reversibility:
not reversible
Remarks on result:
other: observation period: 8 days
Irritation parameter:
conjunctivae score
Basis:
animal #2
Time point:
other: 24 - 72 h
Score:
1
Reversibility:
not reversible
Remarks on result:
other: observation period: 8 days
Irritation parameter:
chemosis score
Basis:
animal #1
Time point:
other: 24 - 72 h
Score:
1.3
Reversibility:
not reversible
Remarks on result:
other: observation period: 8 days
Irritation parameter:
chemosis score
Basis:
animal #2
Time point:
other: 24 - 72 h
Score:
1
Reversibility:
not reversible
Remarks on result:
other: observation period: 8 days
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 24 h to 72 h
Score:
0
Other effects:
Pannus was observed in one animal at the end of the observation period.

Applicant's summary and conclusion

Interpretation of results:
irritating
Remarks:
Migrated information