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There are no long term studies available on the toxicity of Aniline to soil macroorganisms, plants and microorganisms. However, for for plants there are various, valid short term toxicity tests that may be used for a derivation of PNECterrestic.

In EU-Risk Assessment Report (2004), Aniline, terrestrial risk assessment was performed in both, the atmospheric and soil compartment.

To allow the derivation of a PNECpIant for a risk assessment of the atmosphere, a plant fumigation test was performed with aniline (BASF, 2002). Three species of higher plants (Avena sativa, Brassica pekinensis and Abies grandis) were exposed in laboratory exposure chambers for 14 days to 3 aniline concentrations. The lowest NOEC of 0.3 mg/m3 found for Brassica pekinensis is used as basic value for the derivation of PNECplant. An assessment factor of 50 is proposed as the exposure period for the three tested plant species was only 14 days. Therefore PNECplant = 0.3 mg/m3 / 50 = 6 μg/m3.

The toxicity of aniline to Lactuca sativa in natural soil and in nutrient solution was tested in two short term toxicity tests. A 14-day EC50 for growth inhibition of 33 mg/kg soil (dw) and of 56 mg/kg soil (dw) was found. The lowest EC50 value found for soil exposure is used for the determination of the PNEC50. An assessment factor of 1,000 has to be applied. This leads to a PNECsoil of 33 μg/kg (dry weight).

Chronic data on three trophic levels for 3.4 Dichloroaniline were presented in EU-Risk Assessment Report (2004), Aniline. These results indicate that, toxicity of 3.4 TCA to soil organisms is strongly dependent on pre-incubation of the test substance into soil. Significantly higher effect values were observed in tests with soil pre-incubated for 2 -5 weeks than compared to freshly contaminated soils (Table 3.34, p. 54). It can be expected that 3.4 TCA reacts similar in soil as Aniline does, by forming covalent-bonds to humic acids. Under natural conditions, soil organisms will mainly be exposed to the bound substance, as Aniline is set free relatively slowly from the agents and is always in equilibrium with the bound form. Therfore, when performing further terrestrial tests, the binding properties of Aniline should be considered.