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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

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Administrative data

Link to relevant study record(s)

Description of key information

The 72h-EL50 is read across from Lime oil and is 8.0 mg/L and the EL10 is 5.1 mg/l  (Loading rates, WAF study)

Key value for chemical safety assessment

EC50 for freshwater algae:
8 mg/L
EC10 or NOEC for freshwater algae:

Additional information

For lemon oil, no studies on the aquatic toxicity are available for algae. For this endpoint, read-across is conducted to the Citrus group. Key WAF studies are available for orange oil, mandarin oil and lime oil, see summary in the table.

Test substance (Read across)

Endpoint (growth)



Lime Oil Distilled

72h-EL50 = 8 mg/l (WAF)

72h-EL10 = 5.1 mg/l (WAF) 

OECD 201, GLP, Rel. 2, KEY

Harlan 2011

Mandarin Oil

72h-EL50 = 9.7 mg/l (WAF)

72h-EL10 = 7.8 mg/l (WAF) 

OECD 201, GLP, Rel. 2,

Harlan 2011

Lime Oil Cold Pressed

72h-EL50 = 11 mg/l (WAF)

72h-EL10 = 2.2 mg/l (WAF) 

OECD 201, GLP, Rel. 2,

Harlan 2011

Orange Oil

72h-EL50 = 150 mg/l

72h-NOELr = 50 mg/l

OECD 201, GLP, Rel. 2,

Lebertz 2007

The studies are conducted according to OECD 201 and are validated with reliability 1 or 2. For the data gap of lemon oil these studies are considered suitable for read-across and therefore given Reliability 2. The WAF toxicity values (ErL50) for Mandarin oil and both Lime oils are very similar, while for Orange Oil the WAF result showed an ErL50 10 times higher (less toxic).


As the study with Lime oil distilled constitutes the lowest toxicity value (lowest WAF value, highest toxicity) for this category, it was selected as key study. The values for Mandarin oil, the other quality of Lime oil and Orange oil are used as supporting studies.