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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Description of key information

NOEC (21d, overall threshold effects) = 3.22 mg/l (twa)

NOEC (21d, reproduction) = 3.22 mg/l (twa)

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
3.22 mg/L

Additional information

A reproduction test on Daphnia magna (semi-static, 21 days) was carried out to assess the effect of the test item on the reproductive output of Daphnia magna, according to the OECD Guideline 211. Ten daphnids, held individually, were used per concentration level and control. Nominal concentrations of the test item were selected based on the results of a preliminary range finding test (non-GLP) as follows: 0.480 - 1.20 - 3.00 - 7.50 - 18.8 mg/l (separation factor 2.5), corresponding to the time-weighted mean measured test item concentrations 0.540 – 1.29 – 3.22 – 7.78 – 19.3 mg/l. The effects on the reproduction capacity and other test item-related effects or parameters such as the intrinsic rate of the natural increase, first appearance of living juveniles, occurrence of aborted eggs and stillborn juveniles, adult mortality, body length and dry weight of the parental daphnids, were assessed. The test item concentrations were analytically verified via HPLC-DAD in the fresh media at the start of the exposure-renewal intervals on days 0, 9, 14 (0 hours) and in the old media at the end of the exposure intervals on days 2, 12, 16 (48 or 72 hours, respectively) in all concentration levels (with surviving daphnids) and in the control. At the end of the respective exposure-renewal intervals (48 and 72 hours), the measured test item concentrations in the old media were in the range of 97 to 115% of the nominal concentrations. Since the measured test item concentrations were not within ± 20% of the nominal concentrations, the time-weighted mean measured concentrations of the test item were used for evaluation of the effect-concentrations.

NOEC (21d, overall threshold effects) = 3.22 mg/l

NOEC (21d, reproduction) = 3.22 mg/l