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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

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Administrative data

Link to relevant study record(s)

Reference
Endpoint:
toxicity to microorganisms, other
Remarks:
inhibition of bioluminescence
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable, commercially used assay systems
Qualifier:
according to guideline
Guideline:
other: E12-06
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Details on test solutions:
The same stock chemical solution was used to prepare the dilution series for each assay (ToxAlert 10, Microtox and Lumistox, respectively) and all tests were carried out by the same person on the same day.
Test organisms (species):
Vibrio fisheri
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
5 min
Test temperature:
15 °C
pH:
pH 7
Details on test conditions:
Type: aquatic
Reference substance (positive control):
yes
Remarks:
Zinc sulphate in 2% NaCl
Duration:
5 min
Dose descriptor:
EC50
Effect conc.:
12 300 - 17 500 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
growth inhibition

EC50 (5 min) (ToxAlert10): 17500 mg/L
EC50 (5 min) (Microtox): 12900 mg/L
EC50 (5 min) (Lumistox): 12300 mg/L

data related to nominal concentrations

Validity criteria fulfilled:
yes
Conclusions:
The EC50 after a 5 min exposure was determined to range 12300 - 17500 mg/L.
Executive summary:

Jennings et al. (2001) studied the inhibition of the bioluminescence of Vibrio fisheri in presence of DMF. The EC50 after a 5 min exposure was determined as 12300 - 17500 mg/L.

Description of key information

EC50 (5 min): 12300 - 17500 mg/L for Vibrio fisheri (E12-06, static, non-GLP)

Key value for chemical safety assessment

EC50 for microorganisms:
12 300 mg/L

Additional information

Jennings et al. (2001) studied the inhibition of the bioluminescence of Vibrio fisheri in presence of DMF. The EC50 after a 5 min exposure was determined to range 12300 - 17500 mg/L.