Registration Dossier
Registration Dossier
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EC number: 267-097-1 | CAS number: 67786-25-8
Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
Description of key information
LC50 (96 h) Zebra fish > 300 mg/l
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Effect concentration:
- 300 mg/L
Additional information
The short-term toxicity to fish was evaluated in a weight of evidence approach considering data on the substance and on similar substance 3a-MSA. Justification for Read Across is given in Section 13 of IUCLID.
Regarding the study available on 4 -MSA, only the test summary is available. The study was performed on Zebra fish at the dose concentration of 300 mg/l, following the OECD guideline 203 test procedures. No dead occurred, thus the LC50 was established as higher than 300 mg/l (Ciba-Geigy Ltd., 1992). Even if the reliability was set as 4, it is consistent and in agreement with the expected results for the category of Stilbene Fluorescent Whitening Agents and it supports the endpoint in a weight of evidence approach.
The further tests here presented and following a weight of evidence approach, were performed on the analogous 3a-MSA. This substance can be considered the best representative within the category, since it has the same sulphonation degree as 4 -MSA, a very high structural similarity (Tanimoto distance > 0.9) and a very high water solubility (650 g/l Vs 1000 g/l of the substance) (details in the category Justification Report, attached to the Section 13 of the dossier). In all cases, the LD50 was recorded higher than 1000 mg/l.
Furthermore, a prolonged semistatic assay over 14 days was conducted with Brachydanio rerio. Endpoints were mortality, signs of intoxication, length and weight. The nominal concentrations were 100, 316 and 1000 mg/l. There was no mortality and no difference in body weight at nominal concentrations of 100 and 316 mg/l. Only at 316 mg/l one fish showed slackening in movement (Bayer AG., 1992).
In conclusion, no fish acute toxicity is expected for 4 -MSA and the conservative value of LC50 (96h) greater than 300 mg/l, the maximum tested concentration on the substance, has been taken as a reference for the Chemical Safety Assessment.
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