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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to reproduction: other studies
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: acceptable well-documented publication which meets basic scientific principles

Data source

Reference
Reference Type:
publication
Title:
Embryotoxicity of Sixteen Industrial Amines to the Chicken Embryo
Author:
A. Korhonen, K. Hemminki and H. Vainio
Year:
1983
Bibliographic source:
JOURNAL OF APPLIED TOXICOLOGY, VOL. 3, NO. 2, 1983

Materials and methods

Test guideline
Qualifier:
no guideline required
Principles of method if other than guideline:
White Leghorn chicken eggs were treated with triethylamine and observed for signs of toxicity and malformations. The affected embryos were classified into the categories, dysplaying the grade of effects.
GLP compliance:
no
Type of method:
in vivo

Test material

Constituent 1
Chemical structure
Reference substance name:
Triethylamine
EC Number:
204-469-4
EC Name:
Triethylamine
Cas Number:
121-44-8
Molecular formula:
C6H15N
IUPAC Name:
triethylamine
Details on test material:
Triethylamine of technical grade were obtained from Merck, Darmstadt.

Test animals

Species:
other: White Leghorn chicken eggs
Strain:
not specified

Administration / exposure

Route of administration:
other: injection
Type of inhalation exposure (if applicable):
other: not applicable
Vehicle:
acetone
Details on exposure:
no details given
Analytical verification of doses or concentrations:
not specified
Details on analytical verification of doses or concentrations:
no details given
Duration of treatment / exposure:
The solution was injected into the egg in a total volume of 5 µl. Ten control eggs, injected with 5 µL of acetone, were incubated with each batch of eggs. Results from solvent controls were not subtracted from experimental values.
Frequency of treatment:
one-time
Duration of test:
The incubation was terminated 11 days after the injection, after a total incubation of 14 days. The remaining eggs were opened and the embryos inspected for survival and for external malformations.
Doses / concentrations
Remarks:
Doses / Concentrations:
0.5, 1.0, 2.0, 4.0 µmol per egg
Basis:
other: injected
No. of animals per sex per dose:
30 eggs for 0.5, 1.0 and 2.0 µmol; 20 eggs for 4.0 µmol
Control animals:
yes, concurrent vehicle
Details on study design:
no further details on study design available
Statistics:
no details available

Results and discussion

Effect levels

open allclose all
Dose descriptor:
other: ED50
Effect level:
0.9 other: µmol per egg (effect type : embryotoxicity)
Basis for effect level:
other: Malformations: small eye cup, defects of lids and cornea, encephalocoele or skin pimple in head, open coelom, short back or neck and defects of wings
Dose descriptor:
other: LD 50
Effect level:
1.8 other: µmol per egg (effect type embryotoxicity)
Basis for effect level:
other: mortality

Any other information on results incl. tables

The embryotoxic potency of triethylamine including deaths and malformations, is defined by the total effect, i.e. the total number of affected embryos on day 14 of incubation (Table 1). LD50 values for total mortality on day 14 could also be calculated (Table 2).

Table 1. Embryotoxic effects

Treatment (µmol per egg)

Treated embryos
(n)

Early deaths (before day 5)

Late deaths non-malformed embryos

(Days 6-14) malformed embryos

Malformed survivors (on day 14)

All affected embryos

(n)

(%)

(n)

(%)

(n)

(%)

(n)

(%)

(n)

(%)

TEA

 

0.5

30

0

(0)

0

(0)

1

(3)

6

(20)

7

(23)

1.0

30

6

(20)

0

(0)

2

(7)

11

(37)

19

(63)

2.0

30

15

(50)

0

(0)

2

(7)

4

(13)

21

(70)

4.0

20

19

(95)

0

(0)

0

(0)

0

(0)

19

(95)

Table 2. ED50and LD50values of different embryotoxic effects of TEA on chick embryos

 

Total effect ED50(µmol per egg)

95% confidence limits

Total mortality

Early deaths LD50

 

(µmol per egg)

Tanα

(µmol per egg)

Tanα

(µmol per egg)

Tanα

TEA

0.9

(0.65 - 1.2)

0.98

1.6

1.5

1.8

1.2

Estimated from incomplete dose-response curves

Applicant's summary and conclusion

Conclusions:
Triethylamine was one of the most potent chemicals that affected eggs
Executive summary:

Sixteen amine compounds that are used in the rubber industry, including triethylamine were tested on three day chicken embryos for embryotoxicity. The parameters measured were: early deaths recorded within two days of injection, late deaths of malformed embryos, late deaths of non-malformed embryos and malformed survivors. Triethylamine was one of the chemicals which caused the highest frequencies of malformations.