Triethylamine

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: cytogenicity / chromosome aberration

Remarks:

Type of genotoxicity: chromosome aberration

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1971

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well-documented publication which meets basic scientific principles.

Data source

Reference

Reference Type:

publication

Title:

Unnamed

Year:

1971

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

TEA was investigated on male Wistar rats weighing 150-200 g. These were subjected to long-term poisoning by inhalation in 100-liter chambers, predetermined vapor concentrations being maintained around the clock for 3 months.

GLP compliance:

no

Remarks:

The study was conducted prior to the adoption of the OECD guidelines

Type of assay:

chromosome aberration assay

Test material

Test material information

Constituent 1

Reference substance name:

Triethylamine

EC Number:

204-469-4

EC Name:

Triethylamine

Cas Number:

121-44-8

Molecular formula:

C6H15N

IUPAC Name:

triethylamine

Details on test material:

CAS 121-44-8 (triethylamine), purity not indicated.

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

inhalation

Vehicle:

not applicable

Details on exposure:

Rats were exposed by inhalation in 100-L chambers

Duration of treatment / exposure:

30 and 90 days

Frequency of treatment:

daily

Post exposure period:

no

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 1 and 10 mg/m3
Basis:
nominal conc.

No. of animals per sex per dose:

no data

Control animals:

yes, sham-exposed

Positive control(s):

no

Examinations

Tissues and cell types examined:

bone marrow cells

Details of tissue and slide preparation:

The preparation for cytological investigation were prepared by the method of Ford and Woolam (Ford C.E. and Woolam D.H. Stain technology, Vol.38, p.271, 1963).

Evaluation criteria:

The incidence of structural chromosome breakages and aneuploidy, recorded in metaphases of marrow cells, was used as the criterion of a mutagenic effect. The control was provided by the incidence of similar breakages in the marrow of intact rats of the same age and sex, maintained under identical conditions.

Statistics:

Student's test.

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

not specified

Vehicle controls validity:

not applicable

Negative controls validity:

valid

Positive controls validity:

not examined

Additional information on results:

In the rats poisoned with TEA the incidence of cells with structural chromosome breakages did not exceed the control incidence (2%) in any experimental variant, but the incidence of aneuploid cells in the rats exposed to a concentration of 1 mg/m³ was significantly higher than in the controls 30 days after the beginning of poisoning (p<0.01). The incidence of hyperploid cells was similar to the control values in all the experimental groups except those subjected to long-term poisoning with 1 mg/m³ TEA for 30 days (p<0.01).
Rats poisoned with TEA did not exhibit any decrease of mitotic activity in the marrow during the observation period.

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative
TEA do not induce chromosomal breakages in the rat bone marrow cells.

Executive summary:

Male Wistar rats were exposed to 1 and 10 mg/cm³ triethylamine via continuous inhalation for 30 and 90 days. 50 to 100 bone marrow cells were scored per animal. The incidence of cells with chromosomal breakage did not exceed controls but the incidence of aneuploid cells was significantly higher at 1 mg/cm3 after 30 days. There was no incidence of aneupoidy in the dose group exposed to 10 mg/m³ of TEA neither after 30 days nor after 90 days of exposure. No decrease in mitotic activity was observed.