6-phenyl-1,3,5-triazine-2,4-diyldiamine

Acute Toxicity: inhalation

Administrative data

Endpoint:

acute toxicity: inhalation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed according to OECD guideline study with GLP compliance.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1989

Report date:

1989

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 403 (Acute Inhalation Toxicity)

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

6-phenyl-1,3,5-triazine-2,4-diyldiamine

EC Number:

202-095-6

EC Name:

6-phenyl-1,3,5-triazine-2,4-diyldiamine

Cas Number:

91-76-9

Molecular formula:

C9H9N5

IUPAC Name:

6-phenyl-1,3,5-triazine-2,4-diamine

Test animals

Species:

rat

Strain:

Sprague-Dawley

Sex:

male/female

Administration / exposure

Route of administration:

inhalation: aerosol

Type of inhalation exposure:

head only

Vehicle:

other: clean dry filtered compressed air

Analytical verification of test atmosphere concentrations:

yes

Duration of exposure:

4 h

Concentrations:

Single chamber concentration (exposure-dose): 0.687, 1.400, 2.489, 3.365 mg/L by inhalation head only.
the corresponding nominal concentration: 2.726, 6.464, 13.947, 20.167 mg/L

No. of animals per sex per dose:

5

Control animals:

yes

Details on study design:

A similiar group of 10 rats (5 male and 5 female) was exposed to filtered air as a control

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LC50

Effect level:

2.932 mg/L air

95% CL:

> 2.346 - < 4.504

Exp. duration:

4 h

Mortality:

All animals were examined twice daily to detect any which were dead or moribund. Moribund animals were removed and necropsied for human reasons and to prevent autolysis.

Clinical signs:

other: The animals were observed at hourly intervals during the exposure period, then for the remainder of the working day, and once daily thereafter for 14 days.

Body weight:

The body weight of each animal was recorded immediately before and after exposure, on days 8 and 15 of the study and the necropsy.

Gross pathology:

The following procedure were applied to all animals killed at the end of the study and to those killed in extremis.

Necropsy:
A full internal and external examination was made under the general supervision of a pathologist. The nasal passages were examined and an assessment made of any irritation of the respiratory tract.

Organ weights:
The lungs, bronchi and trachea of the animal were dissected free from fat and other contiguous tissue and weighed together.

Histology:
Sample of all gross lesion were fixed in 10% buffered formalin and retained without further processing.

Any other information on results incl. tables

STATISTICAL RESULT:
-death: Deaths occurred in male and female groups at levels of 2.489 mg/L and above. Overall there was a dose-related relationship between  mortality and chamber concentration.  All deaths occurred on days one and two of the study.
-body weight gain: Body weight losses occurred in the first week of the study in exposed groups, with a degree of recovery in the second week.
TOXIC EFFECTS:
-clinical chemistry: Marked clinical signs without any specific signs of local lung toxicity were first observed on the day of exposure.   The signs included lethargy, ataxia and prostation sometimes accompanied by panting.
-histopathology: There was no treatment-related effect on lung weight in survivors, and only occational increases in lung weight in decedents.
-necroscopy: Animals surviving to termination were unremarkable macrocopically.The only changes in decedents were non-specific pulmonary changes.

Concentration(mg/L)       mortality on day       Mortality ratio 
                                          1     2      3-15      days 1-15                               
Male control                                                                              0 / 5
0.687                                                                                        0 / 5
1.400                                                                                        0 / 5
2.489                                         1                                             1 / 5
3.365                                         3                                             3 / 5
Female control                                                                           0 / 5
0.687                                                                                        0 / 5
1.400                                                                                        0 / 5
2.489                                  1     2                                             3 / 5
3.365                                  3                                                    3 / 5

Applicant's summary and conclusion

Interpretation of results:

Toxicity Category IV

Remarks:

Migrated information Harmful if inhaled Criteria used for interpretation of results: EU

Conclusions:

LC50 (4 hr, rat inhalation) = 2.932 mg/L

Executive summary:

In the acute inhalation toxicity study, groups of young adultrat, Aprague-Dawley. male/femalewere exposed by inhalation route to

6 -phenyl-1,3,5 -triazine-2,4 -diyldiamine for 4 hours to head only at concentrations of 0.687, 1.400, 2.489, 3.365 mg/L.  Animals then were observed for 14days.

 

LC50Combined =  2.932  mg/L (95% C.I. 2.346 to 4.504)