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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
migrated information: read-across from supporting substance (structural analogue or surrogate)
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Guideline study, limited documentation, not reliable for the NOEC from a non-verifiable/non-published source.

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Phytotoxicity studies with Lactuca sativa in soil and nutrient solution.
Author:
Hulzebos EM, Adema DMM, Dirven-van Breemen EM, Henzen L, van Dis WA, Herbold HA, Hoekstra JA, Baerselman R, van Gestel CAM
Year:
1993
Bibliographic source:
Environ. Toxicol. Chem. 12, 1079-1094 (1993)
Reference Type:
review article or handbook
Title:
Unnamed
Year:
2008
Title:
Phytotoxicity studies with Lactuca sativa in soil and nutrient solution: IV. Miscellaneous compounds
Author:
Hulzebos EM
Year:
1993
Bibliographic source:
(unpublished data) [cited in EU 2008]

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 208 (Terrestrial Plants Test: Seedling Emergence and Seedling Growth Test)
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Acenaphthene
EC Number:
201-469-6
EC Name:
Acenaphthene
Cas Number:
83-32-9
Molecular formula:
C12H10
IUPAC Name:
1,2-dihydroacenaphthylene
Details on test material:
Acenaphthene
Purity >= 95 %
commercial
Concentration in wash oil: 13 - 15 % w/w [CS 05, GC analysis]

Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
--

Sampling and analysis

Analytical monitoring:
yes
Details on sampling:
--

Test substrate

Vehicle:
yes
Details on preparation and application of test substrate:
- Chemical name of vehicle (organic solvent, emulsifier or dispersant): Acetonic stock solution
- Method of mixing into soil (if used): adsorbed to quartz sand as carrier
- Evaporation of vehicle before use: yes

Test organisms

Species:
Lactuca sativa
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
- Common name: lettuce
- Variety: Lactuca sativa Ravel R2

Study design

Test type:
seed germination/root elongation toxicity test
Study type:
laboratory study
Substrate type:
natural soil
Limit test:
no

Test conditions

Test temperature:
21 ±4 °C
pH:
7.5
Moisture:
Until germination: 25 – 30 % of moisture in soil = about 80 % of the water-holding capacity.
After seed emergence, with the covers removed, water loss was compensated daily by adding demineralised water.
Details on test conditions:
TEST SYSTEM
- Testing facility: Climatic chamber
- Test container (type, material, size): Plastic trays, covered with a glass plate until germination.
- Amount of soil: 250 ml, 400 g soil (humid weight)
- Method of seeding: Ten seeds were sown in 0.25-L plastic trays containing 400 g soil with different concentrations
of the test substances at 25-30% moisture content. The trays were covered with glass plates
until germination of seedlings. After removal of the glass plates, evaporation of water was compensated
daily by adding demineralised water.
Only the first five germinated seedlings were used.
After 7 and 14 d, shoots were harvested by cutting them off at soil level, and the fresh weight was immediately determined.
- No. of seeds per container: 10 seeds/tray = 40 g soil/seed until germination.
- No. of plants (retained after thinning): 5
- No. of replicates per treatment group: 3
- No. of replicates per vehicle control: 3


SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Soil texture (if natural soil): see below

GROWTH CONDITIONS
- Photoperiod: 16 h of light and 8 h of darkness
- Light source: fluorescent tubes
- Light intensity and quality: 6800 lux
- Relative humidity (%): 40 - 80

EFFECT PARAMETERS MEASURED (with observation intervals if applicable): 7 d and 14 d after germination

VEHICLE CONTROL PERFORMED: yes

TEST CONCENTRATIONS
- Spacing factor for test concentrations: nominal concentrations were spaced by a factor of 3.2 (Analytical verification, but not shown)

Results and discussion

Effect concentrationsopen allclose all
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
other: NOEC (normalised to standard soil with 3.4% of organic matter)
Effect conc.:
1.9 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
: Shoot growth based on fresh-weight development
Remarks on result:
other: Adjustment taken from EU 2008
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
other: EC50 (normalised to a standard soil with 3.4% organic matter)
Effect conc.:
47 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
: Shoot growth based on fresh-weight development
Remarks on result:
other: Adjustment taken from EU 2008
Species:
Lactuca sativa
Duration:
14 d
Dose descriptor:
EC50
Effect conc.:
25 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
growth
Remarks:
: Shoot growth based on fresh-weight development
Remarks on result:
other: 95% CL: 16 - 40 mg/kg soil d.w.
Details on results:
--
Results with reference substance (positive control):
no data
Reported statistics and error estimates:
The EC50 was determined by applying a logistic model according to Haanstra et al. 1985.

Applicant's summary and conclusion

Validity criteria fulfilled:
not specified