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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable publication (Ames test at only one concentration level - spot test, no S-9 activation, only S. typhimurium used)

Data source

Reference
Reference Type:
publication
Title:
Study of 106 organic and inorganic compounds in the Salmonella/microsome test.
Author:
De Flora S
Year:
1981
Bibliographic source:
Carcinogenesis 2: 283-298

Materials and methods

Principles of method if other than guideline:
Ames test (spot test, because of the low solubility of the test substance; i.e. placement of 2 mg of the test material in the middle of a plate)
GLP compliance:
no
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Reference substance name:
Molybdenum Orange
IUPAC Name:
Molybdenum Orange
Details on test material:
- Name of test material (as cited in study report): Molybdenum Orange (or lead solfomolybdochromate), industrial grade
- Analytical purity: no data
- Impurities (identity and concentrations): no data
- Composition of test material, percentage of components: PbCrO4, 72-77%; PbSO4, 4-6%; Al2O4, 2%; PbMoO4, 12-14%

Method

Species / strain
Species / strain / cell type:
other: Salmonella typhimurium TA 98, 100, 1535, 1537 and 1538
Metabolic activation:
without
Test concentrations with justification for top dose:
2 mg (spot test)
Vehicle / solvent:
- Vehicle(s)/solvent(s) used: DMSO (at -1.4x10E6 nmoles/plate) or water (not clearly specified)
- Justification for choice of solvent/vehicle: solubility
Controls
Negative solvent / vehicle controls:
yes
True negative controls:
other: addtional compounds (total of 106) were also tested, some showing negative results
Positive controls:
yes
Positive control substance:
other: a couple of polycyclic aromatic hydrocarbons were also tested, including benzo(a)pyrene and benz(a)anthracene
Details on test system and experimental conditions:
spot test according to Ames et al, Mutat Res 31: 347-364; no addtional information
Evaluation criteria:
clearly positive result indicated by a dose- related and reproducible increase of his+ revertant over controls (at least a 3-fold increase ); weak positivity indicated by an increase of revertants 2 to 3 times the controls; ambiguous response indicated by reproducible but less than 2-fold increase of revertants; negative result indicated by no conclusive experiment carried out with the corresponding strain.
Statistics:
no data

Results and discussion

Test results
Metabolic activation:
without
Genotoxicity:
positive
Remarks:
only TA 100
Cytotoxicity / choice of top concentrations:
not specified
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: other: Salmonella typhimurium TA 98, 100, 1535, 1537 and 1538
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

The test substance proved positive only in S. typhimurium TA100 strain.

    

It is also stated that the test substance was positive in the plate test, when solubilized in 0.5 M sodium hydroxide (statement without any further specification, for example concerning the bacterial strains used).

Applicant's summary and conclusion