1,4-dichlorobenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: GLP declaration given but no certificate

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1987

Report date:

1987

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

GLP compliance:

yes

Test type:

standard acute method

Limit test:

yes

Test material

Test material information

Constituent 1

Reference substance name:

1,4-dichlorobenzene

EC Number:

203-400-5

EC Name:

1,4-dichlorobenzene

Cas Number:

106-46-7

Molecular formula:

C6H4Cl2

IUPAC Name:

1,4-dichlorobenzene

Test material form:

solid: flakes

Details on test material:

p-dichlorobenzene, lot: 08146-P
content 99.9 %
Paradichlorobenzene was prepared at 20% (w/v) concentration in corn oil and administered at a volume of 10.0 ml/kg.
Stability and absorbtion of the test substance were not determined.

Test animals

Species:

rat

Strain:

other: CFY ( Sprague-Dawley origin)

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Age at study initiation: 4-6 weeks
- Weight at study initiation: 103-145 g
- Housing: in groups
- Diet ad libitum
- Water ad libitum
- Acclimation period: 6 days

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 21-22
- Humidity (%): 54
- Air changes (per hr): 15
- Photoperiod (hrs dark / hrs light): 12/12

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

corn oil

Details on oral exposure:

Single oral application by gavage as 20% (w/v) concentration in corn oil and administered at a volume of 10.0

Doses:

single dose of 2000 mg/kg bodyweight.

No. of animals per sex per dose:

5 F
5 M

Control animals:

no

Details on study design:

All animals were observed 14 days after dosing and then sacrificed and subjected to macroscopic examination.

Statistics:

no

Results and discussion

Effect levels

Effect levels 1

Sex:

male/female

Dose descriptor:

LD0

Effect level:

> 2 000 mg/kg bw

Remarks on result:

other: no animal died;

Effect levels 2

Sex:

male/female

Dose descriptor:

LD50

Effect level:

> 2 000 mg/kg bw

Remarks on result:

other: no animal died during the observation period and recovered from unspecific findings (piloerection, hunched posture) by day 3

Mortality:

no animal died

Clinical signs:

other: Signs of reaction to treatment observed in rats doses orally with paradichlorbenzene: Signs No.of rats in group of 5 showing signs Male

Gross pathology:

Terminal ausopsy findings were normal.

Any other information on results incl. tables

There were no deaths following a single oral dose of paradichlorobenzene at 2000 mg/kg bodyweigt.

Applicant's summary and conclusion

Executive summary:

In a study according OECD Guideline 401 (Acute Oral Toxicity) and GLP 10 male and female rats were tested of aute toxicity receiving 2000 mg/kg bw by gavage. Within 3 hours post application the rats displayed

salivation, piloerection, hunched posture and abnormal gait but recovered within 3 days. No animal died during the 14 day observation period. The acute lethal dose of paradichlorobenzene in rat was found to be greater than 2000 mg/kg bodyweight .