Registration Dossier
Registration Dossier
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EC number: 215-251-3 | CAS number: 1314-98-3
Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro DNA damage and/or repair study
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Study period:
- Not reported
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Document insufficient for assessment.
- Justification for data waiving:
- other:
Cross-referenceopen allclose all
- Reason / purpose for cross-reference:
- reference to same study
- Reason / purpose for cross-reference:
- reference to other study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 970
Materials and methods
- Principles of method if other than guideline:
- Method: Induction of mitotic gene conversion or non-reciprocal recombination was studied with the diploid strain D4 of Saccharomyces cerevisiae.
- GLP compliance:
- not specified
- Type of assay:
- mitotic recombination assay with Saccharomyces cerevisiae
Test material
- Reference substance name:
- Zinc sulphate
- EC Number:
- 231-793-3
- EC Name:
- Zinc sulphate
- Cas Number:
- 7733-02-0
- IUPAC Name:
- zinc sulfate
- Details on test material:
- - Name of test material (as cited in study report): Zinc sulfate
Constituent 1
Method
- Target gene:
- Gene conversion was studied at the following two loci: ade2 and trp5.
Species / strain
- Species / strain / cell type:
- Saccharomyces cerevisiae
- Details on mammalian cell type (if applicable):
- Not applicable
- Additional strain / cell type characteristics:
- not applicable
- Metabolic activation:
- not applicable
- Metabolic activation system:
- Not applicable
- Test concentrations with justification for top dose:
- Optimal or highest concentration (ppm): 5,000/1,000
- Vehicle / solvent:
- 0.1M potassium phosphate buffer (pH 7.5)
Controls
- Untreated negative controls:
- not specified
- Negative solvent / vehicle controls:
- yes
- Remarks:
- 0.1 M potassium phosphate buffer (pH 7.5)
- True negative controls:
- not specified
- Positive controls:
- not specified
- Positive control substance:
- not specified
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in suspension
DURATION
- Exposure duration: 4 h - Evaluation criteria:
- No data
- Statistics:
- No data
Results and discussion
Test results
- Species / strain:
- Saccharomyces cerevisiae
- Metabolic activation:
- not applicable
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- not determined
- Vehicle controls validity:
- not applicable
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- not applicable
- Additional information on results:
- None
- Remarks on result:
- other: strain/cell type: diploid strain D4
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Table 1: Zinc sulfate tested for the induction of mitotic gene conversion in strain D4 of Saccharomyces cerevisiae
Convertants per 106survivors at the loci |
Survivors (%) |
Optimal or highest concentration (ppm) |
|
ade2 |
trp5 |
||
45.7 (28.5) |
2.9 (3.3) |
97 |
5000/1000 |
Doses are presented at which the highest frequencies of mitotic gene convertants per survivor were observed. Those concentrations were sometimes different for conversion at the two loci. The corresponding control values are given in parentheses. Treatments were performed in 0.1M potassium phosphate butter (pH 7.5) with shaking for 4 h at 25 ± 0.1 °C.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The test material was considered to be non-convertogenic under the conditions of this test. - Executive summary:
A study was conducted to determine the potential of the test material to induce mitotic gene conversion using diploid strain D4 of Saccharomyces cerevisiae.
Diploid strain D4 (MD 20:a,+,ade2-I, trp5-27-+) was treated with the test material for 4 h in selective synthetic media (WICKERHAM), supplemented with amino acids and nucleobases (ROMAN) and solidified with 1.5% Difco bactoagar, usually up to 5,000 ppm/plate.
The test material showed inability to induce mitotic gene conversion. Convertants per 106survivors at the loci ade2 & trp5 was 45.7 & 2.9, respectively.
The test material was considered to be non-convertogenic under the conditions of this test.
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