Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Endpoint:
activated sludge respiration inhibition testing
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: The study was conducted according to the appropriate OECD test guideline and in compliance with GLP. The restriction is that

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
2002
Report date:
2002

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 209 (Activated Sludge, Respiration Inhibition Test
GLP compliance:
yes (incl. QA statement)

Test material

Constituent 1
Chemical structure
Reference substance name:
(3-chloro-2-hydroxypropyl)trimethylammonium chloride
EC Number:
222-048-3
EC Name:
(3-chloro-2-hydroxypropyl)trimethylammonium chloride
Cas Number:
3327-22-8
Molecular formula:
C6H15ClNO.Cl
IUPAC Name:
3-chloro-2-hydroxy-N,N,N-trimethylpropan-1-aminium chloride
Details on test material:

69.35 % 3-Chloro-2-hydroxypropyl)trimethylammoniumchloride, 0.2 % 2,3-Dihydroxypropyltrimethylammoniumchloride, <0.05 % 2.3-epoxypropyltrimethylammoniumchloride, 28.85 % water

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
no

Test organisms

Test organisms (species):
activated sludge of a predominantly domestic sewage

Study design

Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h

Test conditions

Details on test conditions:
Type: aquatic

Results and discussion

Effect concentrationsopen allclose all
Duration:
3 h
Dose descriptor:
EC10
Effect conc.:
1 032 mg/L
Duration:
3 h
Dose descriptor:
EC50
Effect conc.:
> 2 000 mg/L
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)
Duration:
3 h
Dose descriptor:
other: EC20
Effect conc.:
> 2 000 mg/L
Remarks on result:
other: IUCLID4 note: "m" (measured/nominal)

Any other information on results incl. tables

No EC50-value could be found with the highest test concentration of 2000 mg/l. In the test report EC20-, EC50 -
and EC80-values higher than 2000 mg/l have been reported, but no EC10-value has been estimated. Based on graphical
estimation EC10-value of 1225 mg/l can be extrapolated.
There were no estimations of goodness of fit parameters available in graphs (standard deviation, R2 etc.)

In the addendum to the test report EC10-value has been estimated to be 1032 mg/l.

Applicant's summary and conclusion

Validity criteria fulfilled:
yes
Conclusions:
In a good quality study conducted according to OECD Test Guideline 209 and in compliance with GLP, the EC50 for respiration inhibition of activated sludge was determined to be >2000 mg/l. This result is expressed as active ingredient.