alkyl 2-alkyl-1,2,3,4,4a,5,8,8a-octahydro-1,4:5,8-dimethanonaphthalene-2-carboxylate

Biodegradation in water: screening tests

Administrative data

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: The study was performed according to the recommended guidelines and GLP, and without deviations.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2008

Report date:

2008

Materials and methods

Test guideline

Test guideline 1

Qualifier:

according to guideline

Guideline:

EU Method C.4-C (Determination of the "Ready" Biodegradability - Carbon Dioxide Evolution Test)

Test guideline 2

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

Test guideline 3

Qualifier:

according to guideline

Guideline:

ISO DIS 9439 (Ultimate Aerobic Biodegradability - Method by Analysis of Released Carbon Dioxide)

GLP compliance:

yes

Test material

Reference

Name:

Unnamed

Type:

Constituent

Study design

Oxygen conditions:

aerobic

Inoculum or test system:

other: activated sludge freshly obtained from a municipal sewage treatment plant

Details on inoculum:

Source: the source of test organisms was activated sludge freshly obtained from a municipal sewage treatment plant: 'Waterschap de Maaskant', 's-Hertogenbosch, The Netherlands, receiving predominantly domestic sewage.

Treatment: The freshly obtained sludge was kept under continuous aeration until further treatment. The concentration of suspended solids was 3.3 g/l in the concentrated sludge (information obtained from the municipal sewage treatment plant). Before use, the sludge was allowed to settle (43 minutes) and the liquid was decanted for use as inoculum at the amount of 10 ml/l of mineral medium.

Duration of test (contact time):

28 d

Initial test substance concentration

Initial test substance concentration 1

Initial conc.:

13 mg/L

Based on:

test mat.

Initial test substance concentration 2

Initial conc.:

10 mg/L

Based on:

other: TOD

Parameter followed for biodegradation estimation

Parameter followed for biodegradation estimation:

CO2 evolution

Reference substance

Reference substance:

acetic acid, sodium salt

Results and discussion

% Degradation

Parameter:

% degradation (CO2 evolution)

Value:

>= 3 - <= 10

Sampling time:

29 d

Details on results:

Points of degradation plot (test substance):
3 % degradation after 29 d for bottle 1
10 % degradation after 29 d for bottle 2

Any other information on results incl. tables

 

Day	Test substance %	Day	Reference substance %
0	0	0	0
2	1	2	16
5	3	5	41
7	4	7	55
9	4	9	62
14	5	14	72
19	6	19	78
23	6	23	80
27	6	27	81
29	6	29	82
29	6	29	82
29	7	29	82

 

Applicant's summary and conclusion

Validity criteria fulfilled:

yes

Interpretation of results:

other: not readily biodegradable

Conclusions:

The test substance was found not to inhibit microbial activity and was not readily biodegradable under the test conditions.
There were no deviations from guideline; validity criteria have been fulfilled.