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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
1 (reliable without restriction)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1996
Report date:
1996

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
other: 92/69/EEC (Ames)
GLP compliance:
yes
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
2,2,4-trimethyl-4-phenyl-butane-nitrile
EC Number:
422-580-8
EC Name:
2,2,4-trimethyl-4-phenyl-butane-nitrile
Cas Number:
75490-39-0
Molecular formula:
C13 H17 N
IUPAC Name:
2,2-dimethyl-4-phenylpentanenitrile

Method

Species / strain
Species / strain / cell type:
bacteria, other: Salmonella typhimurium TA1535, TA1537, TA1538, TA98, TA100 and Escherichia coli WP2 uvrA
Metabolic activation system:
Aroclor-induced rat liver S9
Test concentrations with justification for top dose:
Concentration range in the main test (with metabolic activation): 156.25 ... 5000 µg/plate
Concentration range in the main test (without metabolic activation): 156.25 ... 5000 µg/plate
Vehicle / solvent:
Solvent: Dimethylsulfoxide

Results and discussion

Test resultsopen allclose all
Species / strain:
other: as specified above
Metabolic activation:
with
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
( 5000 µg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
( 5000 µg/plate)
Species / strain:
other: as specified above
Metabolic activation:
with
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
( 2500 µg/plate)
Species / strain:
other: as specified above
Metabolic activation:
without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
cytotoxicity
Remarks:
( 2500 µg/plate)
Additional information on results:
Observations:
No increases in revertant colony numbers of any of the
tester strains were observed following treatment at any dose
level, in the presence or absence of S-9 mix. The solvent
control plates gave counts in the expected ranges. All
positive controls produced marked increases in the revertant
counts.
Remarks on result:
other: other: preliminary test
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative with metabolic activation
negative without metabolic activation