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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

endocrine system modulation
Type of information:
experimental study
Adequacy of study:
other information
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study which meets generally accepted scientific standards

Data source

Reference Type:
Screening of some anti-androgenic endocrine disruptors using a recombinant cell-based in vitro bioassay
Roy P, Salminen H, Koskimies P, Simola J, Smeds A, Saukko P & Huhtaniemi IT
Bibliographic source:
PMID: 15084347, J Steroid Biochem Mol Biol. 88(2):157-66

Materials and methods

Test guideline
no guideline followed
Principles of method if other than guideline:
Cell-based androgen reporter assay using the Chinese hamster ovarian cell line (CHO K1) in the 96 -well format. The purpose of this assay is a high throughput screening for endocrine disrupting (ED) substances in environmental and biological samples.
CHO cells were cotransfected with plasmids encoding mouse mammary tumour virus-neomycin-luciferase and human androgen receptor (hAR). A stable cell line was established. After selection with neomycin, a highly active clone was obtained which stably expressed both the hAR and the androgen-responsive luciferase reporter.
GLP compliance:
Type of method:
in vitro

Test material

Constituent 1
Chemical structure
Reference substance name:
Bis(4-chlorophenyl) sulphone
EC Number:
EC Name:
Bis(4-chlorophenyl) sulphone
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): 4,4'-Dichlorodiphenyl sulphone

Administration / exposure

Analytical verification of doses or concentrations:
Details on analytical verification of doses or concentrations:
The concentrations of the stock solutions of all the test compounds were 1 mmol/L in ethanol. They were then further diluted in the medium, resulting in the final concentration of ethanol in the incubations of 0.01%. Thus the final concentration was 0.01/100 mmol/L = 0.0001 mmol/L = 0.1 µmol/L.
Doses / concentrations
Doses / Concentrations:
0.1 µmol/L = 0.287 ng/L for anti-androgenic effects up to 50 µmol/L
nominal conc.


stimulation of luciferase activity
Positive control:
R1881 (Metribolone CAS 965-93-5)

Results and discussion

Details on results:
Test result:
- DCDPS incubation to the in vitro asssay resulted in a negative response. Therefore no androgenic or anti-androgenic effects were identified.

Validity of the assay:
- the test system was idendified as specific and functioning, since stimulation of the cells with androgens for 24h resulted in about 15-fold stimulation of luciferase activity. Furthermore potent steroidal and non-steroidal anti-androgens significantly inhibited the androgen-induced transactivation.
- non-androgenic steroids showed weak activity at high concentrations.
- RT-PCR and western blot confirmed proper transcription and translation as well as stable expression of the AR gene in the cells.

Applicant's summary and conclusion