Registration Dossier
Registration Dossier
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EC number: 201-126-0 | CAS number: 78-59-1
Toxicity to terrestrial plants
Administrative data
- Endpoint:
- toxicity to terrestrial plants: short-term
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The relative phytotoxicity of selected hydrocarbon and oxygenated solvents and oils
- Author:
- Krenek MR and King DN
- Year:
- 1 987
- Bibliographic source:
- Am. Soc. Test. Mater. Spec. Tech. Publ. (Philadelphia PA): Pesticide formulations and application systems: sixth volume
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Undiluted solvents were applied once at a nominal concentration of 32.7 l/ha in a spray booth to approximately 2 week old postemergent plants (cotton, soybean, corn, wheat) in a greenhouse. There were two replicates for each treatment and observations for phytotoxic effects were made at varied intervals over a 56-hour period.
- GLP compliance:
- no
Test material
- Reference substance name:
- 3,5,5-trimethylcyclohex-2-enone
- EC Number:
- 201-126-0
- EC Name:
- 3,5,5-trimethylcyclohex-2-enone
- Cas Number:
- 78-59-1
- Molecular formula:
- C9H14O
- IUPAC Name:
- 3,5,5-trimethylcyclohex-2-enone
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Details on preparation and application of test substrate:
- STOCK AND TEST SOLUTION AND THEIR PREPARATION
- Vehicle, solvent: undiluted, i.e. no vehicle / solvent
- The test item was applied using a spray booth at a controlled nominal rate of 32.7 L/ha
Test organisms
open allclose all
- Species:
- Triticum aestivum
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- Wheat, strain: Sturdy
- Age: 12-14 days after emergence
- Species:
- Zea mays
- Plant group:
- Monocotyledonae (monocots)
- Details on test organisms:
- Corn, strain: H-TAM50
- Age: 12-14 days after emergence
- Species:
- Glycine max (G. soja)
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Soybean, strain: Hutton
- Age: 12-14 days after emergence
- Species:
- other: Gossipium sp.
- Plant group:
- Dicotyledonae (dicots)
- Details on test organisms:
- Cotton; strain: Stoneville 825
- Age: 12-14 days after emergence
Study design
- Test type:
- vegetative vigour test
- Study type:
- semi-field study
- Substrate type:
- not specified
- Limit test:
- no
- Total exposure duration:
- 56 h
Test conditions
- Details on test conditions:
- TEST SYSTEM
- Test type: one-time spray application "over the top"
- Dosing rate: 3.27 isophorone ml/m2, undiluted
- Exposure vessel type: greenhouse after application
- Number of replicates: 2
- Watering: dist. water with or without 0.33 ml/l "Peters professional soluble plant food, grade 20-20-20)
ENDPOINTS ASSESSED
- percentage of leaf surface affected using a scale of 0 to 100, with the untreated controls rated 0
- The 0-100 ratinges were converted to a simplified classification from A (minimum) to D (maximum effects)
- observation after 4, 8, 24, 32, 48, 56 hours - Nominal and measured concentrations:
- Application rate: 32.7 L/ha
- Reference substance (positive control):
- yes
- Remarks:
- xylene range aromatic solvent
Results and discussion
- Details on results:
- Effect of Isophorone on various crops
- corn: class B; max.damage after 4 h (12.5 %)
- wheat: class B; max.damage after 8-56 h (10 %)
- cotton: class B; max.damage after 4 / 24 h (35 %)
- soybean: class D; max. damage after 4 h (50 %)
No plant died, but slight effects were observed on corn, wheat and cotton and maxium effects were observed on soybeans (50 % of the leafes were
damaged). Maximum damage in the overall study was observed after 32 h. Recovery and renewed growth was observed after 56 h. No solvent was
significantly more phytotoxic than the reference solvent. Low phytotoxicity was observed with non-polar solvents. High phytotoxicity was observed
with aromatic solvents. The effects of surface tension (limiting contact to leaf surface) and solution potential for wax layer of leaves are discussed.
For clarification further studies would be needed.
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
