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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Genetic toxicity: in vitro

Currently viewing:

Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
key study
Study period:
not applicable
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: reliable with some restriction: method similar to OECD/EU test method, chemical: no purity reported; individual plate counts not known; no statistics
Cross-referenceopen allclose all
Reason / purpose for cross-reference:
reference to same study
Reason / purpose for cross-reference:
reference to other study

Data source

Reference
Reference Type:
publication
Title:
Unnamed
Year:
1986

Materials and methods

Test guidelineopen allclose all
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 471 (Bacterial Reverse Mutation Assay)
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.13/14 (Mutagenicity - Reverse Mutation Test Using Bacteria)
GLP compliance:
not specified
Type of assay:
bacterial gene mutation assay

Test material

Constituent 1
Reference substance name:
Cadmium oxide
EC Number:
215-146-2
EC Name:
Cadmium oxide
Cas Number:
1306-19-0
IUPAC Name:
oxocadmium
Details on test material:
name of test substance: cadmium oxide

Method

Target gene:
not applicable
Species / strain
Species / strain / cell type:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Details on mammalian cell type (if applicable):
not applicable
Additional strain / cell type characteristics:
not specified
Metabolic activation:
with and without
Metabolic activation system:
Aroclor 1254-induced rat and hamster metabolic activation system
Test concentrations with justification for top dose:
0, 3.3, 10.0, 33.0, 100.0, 333.0, 1000.0, 3333.0 µg/plate
3 plates per dose level
Vehicle / solvent:
Dimethylsulfoxide
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
yes
True negative controls:
no
Positive controls:
yes
Positive control substance:
no
Remarks:
none
Details on test system and experimental conditions:
plate method of Ames (plate incorporation)
endpoint: gene mutation
Evaluation criteria:
ASSESSMENT CRITERIA: a reproducible and dose-related increase in the number of revertants over background even if the increase was less than twofold is considered to be a positive result. When the chemical elicited no increase in the number of revertants: non-mutagenic response. When there as an absence of a clear-cut dose-related increase in revertants; when the dose-related increase were not reproducible or when the response was of insufficient magnitude to support a determination of mutagenicity, the response was considered questionable.
Statistics:
no statistics reported

Results and discussion

Test results
Species / strain:
S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
Metabolic activation:
with and without
Genotoxicity:
negative
Remarks:
in vitro gene mutation
Cytotoxicity / choice of top concentrations:
no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
Vehicle controls validity:
valid
Untreated negative controls validity:
valid
Positive controls validity:
valid
Additional information on results:
none
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Any other information on results incl. tables

none

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative

This study reported that CdO, dissolved in DMSO is not mutagenic in the used test system (Salmonella typhimurium) with or without metabolic activation.
Executive summary:

Mortelmans et al. (1986) reported that cadmium oxide was negative in Salmonella typhimurium with and without metabolic activation tested from 3.3-3333 μg CdO/plate.