1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylindeno[5,6-c]pyran

Monitoring data

Administrative data

Endpoint:

monitoring data

Type of information:

experimental study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

test procedure in accordance with generally accepted scientific standards and described in sufficient detail

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2012

Report date:

2012

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

In 2011, concentrations of HHCB were measured in sewage treatment plants in Berlin, Germany, at two points in time and in sediment cores of the Teltow Canal in Berlin.

GLP compliance:

no

Type of measurement:

other: Cconcentrations in sewage treatment plants and sediment cores

Media:

other: sludge and sediment

Test material

Test material information

Constituent 1

Reference substance name:

1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylindeno[5,6-c]pyran

EC Number:

214-946-9

EC Name:

1,3,4,6,7,8-hexahydro-4,6,6,7,8,8-hexamethylindeno[5,6-c]pyran

Cas Number:

1222-05-5

Molecular formula:

C18H26O

IUPAC Name:

4,6,6,7,8,8-hexamethyl-1H,3H,4H,6H,7H,8H-indeno[5,6-c]pyran

Test material form:

liquid: viscous

Study design

Details on sampling:

STP
Samples were taken from the following sewage treatment plants:
- The STP Wassmansdorf is located about 3 km downstream of the river Dahme in the area of Rudow1). STP Wassmansdorf has an average dry weather flow of 192.900 m3 per day and serves a population of about 1.2 million inhabitants;
- The STP Ruhleben has an average dry weather flow of 180.000 m3 per day) and serves a population of 1.2 million inhabitants. This plant discharges to the Teltow Canal only during the summer season; during winter the discharge is directly into the river Havel in the north of Berlin;
- The STP Schönerlinde receives the major part of its water from the North-eastern part of Berlin, from the Prenzlauer Berg to Marzahn and Pankow. The plant has an average dry weather flow of 100,000 m3 per day and serves a population of circa 0.75 million inhabitants. The water is discharged into the Nordgraben and next it flows into the Tegeler

Samples were taken from the activated sludge tank according to a well-described protocol and prepared for transport by freeze-drying.

SEDIMENT
Sediment samples were taken by Mathias Ricking from the group Earth Sciences, Hydrogeology and Geochemistry of the Free University of Berlin. The Teltow Canal in Berlin receives the treated sewage water of the Berlin metropolis almost without any dilution. Practically the canal acts as a settling area for residual sludge particles that are discharged by the various sewage treatment plants in Berlin, and thus the canal may be considered as an example of a ‘realistic worst case’ with respect to the load of fragrance ingredients.They were taken from the top layer of organic-rich fine-grained material (0-5 cm) on 7 sites along the Teltow Canal, up- and downstream of the sewage treatment plants. These were the same sites as sampled in the 2004 program. In some cases a top layer of sandy material (5-6 cm) was on top of the cores, due to the opening of the Canal for heavier ships after 2005. The age of the layer was estimated to be deposited during 2005-2008, maybe 2009. They were definitely younger than the samples taken in the previous program. The samples were taken on 30 May and 1 June 2011. During the sampling procedure the samples are deep frozen and they were stored frozen. A delay in the freeze-drying procedure was reported on 10 June. The control sample with silica gel was spiked just before the freeze/drying. After freeze-drying the samples were stored at 4ºC before being sent to Omegam Laboratories where they arrived on July 14. They were extracted on 21 July.

Results and discussion

Details on results:

Concentrations on sludge standardized to 35% organic carbon content
- Ruhleben (18/4/2011): 9.3 mg/kg dw
- Ruhleben (23/5/2011): 8.5 mg/kg dw
- Schönerlinde (18/4/2011): 11 mg/kg dw
- Schönerlinde (23/05/2011): 7.8 mg/kg dw
- Wassmansdorf (18/4/2011): 12 mg/kg dw
- Wassmansdorf (23/05/2011): 12 mg/kg dw


Concentrations on sediment standardized to 5% organic carbon content
- 0.5 km downstream outlet STP Wassmansdorf: 0.86 mg/kg dw
- 1.0 km downstream outlet STP Wassmansdorf: 0.34 mg/kg dw
- 1.5 km downstream outlet STP Wassmansdorf: 0.27 mg/kg dw
- Griebnitzsee, near entrance to river Havel: 0.27 mg/kg dw



The standardized concentrations in the sediment for HHCB show no relation to distance from the STP. This relation was apparent before standardization. The sample from the Griebnitzsee also shows elevated concentrations of HHCB in combination with a high level of organic carbon. The origin of this organic load is not known.

Any other information on results incl. tables

Sludge from STPs: Quality control: Local controls
- Local blank (18 April 2011): 1.06 mg/kg dw (172% recovery)
- Local blank (23 May 2011): <0.1 mg/kg dw (113% recovery)
- In general local blank values were at or below 0.1 mg/kg. However, in particular in the first batch, elevated levels were found for HHCB. At the same time the local recovery in the first batch showed an elevated recovery for HHCB.

 

Sludge from STPs: Quality control: Analytical controls
- Omegam blank (batch 18 April 2011): 1 mg/kg dw (116% recovery)
- Omegam blank (batch 23 May 2011): 0 mg/kg dw (101% recovery)

 

Sludge from STPs: Concentrations in sludge of STPs (Non-standardized for Organic carbon)
- Berlin (18 April 2011): 9.3 (39% Organic carbon), 11 (38% Organic carbon), and 12 mg/kg dw (46% Organic carbon) at Ruhleben, Schönerlinde, and Wassmansdorf, respectively.
- Berlin (23 May 2011): 8.5 (44% Organic carbon), 7.8 (35% Organic carbon), and 12 mg/kg dw (47% Organic carbon) at Ruhleben, Schönerlinde, and Wassmansdorf, respectively.
- The mean values for HHCB are 10.8 and 9.4 mg/kg (Local Blank 1 mg/kg). When the results are used without correction for the contamination, the error is circa 10 - 12 %. For an indication of the trend this does not seem to be very relevant.

 

Sediment: Quality control: Local controls
- Local blank: <0.1 mg/kg dw (104% recovery)

 

Sediment: Quality control: Analytical controls
- Local blank: 1 mg/kg dw (116% recovery)

 

Sediment: Concentrations in sediment 0-5 cm upper layer samples upstream and downstream of the outlet of STP Wassmannsdorf in the Teltow Canal in Berlin (30 May 2011) (Non-standardized for Organic carbon)
- 0.1 km upstream: <0.1 mg/kg dw (1.6% Organic carbon)
- 0.5 km downstream: 0.86 mg/kg dw (6.8% Organic carbon)
- 1.0 km downstream: 0.34 mg/kg dw (2.5% Organic carbon)
- 1.5 km downstream: 0.27 mg/kg dw (2.4% Organic carbon)

Sediment: Concentrations in sediment samples taken in the Teltow Canal and in the Griebnitzsee, Berlin (1 June 2011) (Non-standardized for Organic carbon)
- 1 km downstream outlet STP Ruhleben: <0.1 mg/kg dw (1.3% Organic carbon)
- 1 km downstream outlet STP Stahnsdorf: <0.1 mg/kg dw (0.38% Organic carbon)
- Griebnitzsee, near entrance to river Havel: <0.1 mg/kg dw (6.9% Organic carbon)

Applicant's summary and conclusion

Conclusions:

The concentration of HHCB in sludge in Berlin ranged from 7.8 to 12 mg/kg dw.

Executive summary:

In 2011, the concentrations HHCB were measured in sewage treatment plants (STPs) in Berlin, Germany, at two points in time and in sediment cores of the Teltow Canal in Berlin. 

The sludge samples were taken in the STPs in Ruhleben, Schönerlinde and Wassmannsdorf. Samples were taken from the activated sludge tank according to a well-described protocol and prepared for transport by freeze-drying. The analyses were performed in Amsterdam by Omegam. 

The Teltow Canal in Berlin receives the treated sewage water of the Berlin metropolis almost without any dilution. Practically the canal acts as a settling area for residual sludge particles that are discharged by the various sewage treatment plants in Berlin, and thus the canal may be considered as an example of a ‘realistic worst case’ with respect to the load of fragrance ingredients. Samples were taken of the top layer of organic-rich fine-grained material (0-5 cm) on 7 sites along the Teltow Canal, up- and downstream of the sewage treatment plants.

The concentration HHCB in sludge ranged from 9.3 to 12 mg/kg dw on April, 18th and 7.8 to 12.0 mg/kg dw on May 30th/June 1st. For HHCB, the local recovery in the sludge samples was 172%, while the expected recovery range is 85-125%. Therefore the April result was disregarded. 

The concentration HHCB in sediment ranged from 0.2 to 1.1 mg/kg dw.

In 2000 and 2003/2004, similar monitoring activities have been carried out and the current results were compared to the earlier results for HHCB. The concentration of HHCB in sludge remained at the same level. The concentrations of HHCB  in sediment seemed to decrease slightly.