Registration Dossier

Data platform availability banner - registered substances factsheets

Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Toxicity to microorganisms

Currently viewing:

Administrative data

Link to relevant study record(s)

toxicity to microorganisms, other
NOEC derived from a ready biodegradability test according to OECD TG 301C.
Type of information:
experimental study
Adequacy of study:
key study
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
according to guideline
other: OECD Guideline 301C (Ready Biodegradability: Modified MITI Test (I))
Version / remarks:
From this study a NOEC may be derived as the substance is determined to be 'readily biodegradable'.
GLP compliance:
not specified
probably yes
Details on test solutions:
In the ready biodegradability study 100 mg/L test substance was inoculated with 30 mg/L activated sludge.
Test organisms (species):
activated sludge
Details on inoculum:
Although no specifics on used inoculum are reported, normally in Japanese studies a mixture of sewage, soil and natural water is used.
Test type:
Water media type:
Total exposure duration:
14 d
Nominal and measured concentrations:
- Nominal concentration: 100 mg/L
Key result
14 d
Dose descriptor:
Effect conc.:
100 mg/L
Nominal / measured:
Conc. based on:
test mat.
Basis for effect:
other: ready biodegradability
Details on results:
After 14 days inoculation of 100 mg of the substance with activated sludge, 80-90% biodegradation was observed and the substance assessed as readily biodegradable.

Description of key information

The NOEC is set at 100 mg/L based on the substance being ready biodegradability at this concentration in a screening study according to OECD TG 301C.

Key value for chemical safety assessment

Additional information

No activated sludge respiration inhibition test according to OECD TG 209 is available. However, in a biodegradation screening study according to OECD TG 301C, the substance was found to be readily biodegradable at a test substance concentration of 100 mg/L. According to REACH guidance R7.b, this concentration may be considered as a NOEC value for STP microorganisms.

The low toxicity is further supported by data available from a bacterial growth inhibition test according to DIN 38412. In this study Pseudomonas putida was exposed to nominal concentrations of 156.25, 312.5, 625, 1250, 2500, 5000, and 10000 mg/L mg/L for 17 hours under static conditions. The 17-h EC50 and EC10 values were determined at 468 mg/L and 337 mg/L, respectively.