2-(3-(4-amino-9,10-dihydro-3-sulpho-9,10-dioxoanthracen-4-yl)aminobenzenesulphonyl)vinyl) disodium sulphate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Qualifier:

according to guideline

Guideline:

EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Melting point: > 320°C
- Boiling point: NA
- Vapour pressure: NA
- Henry's law constant (for volatie substances): NA
- Water solubility (under test conditions): ca 171 g/l (corrected for purity)
- Solubility in organic solvents: NA
- log Pow: ca. 7.1

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Kommunale Kläranlage Frankfurt-Sindlingen
- Preparation of inoculum for exposure:
- Pretreatment: according to directive section 1.6.3.1
- Concentration of sludge: according to directive section 1.6.4

Duration of test (contact time):

28 d

Initial conc.:

110 mg/L

Based on:

test mat.

Initial conc.:

39.02 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Parameter:

% degradation (DOC removal)

Value:

30 - 40

Sampling time:

28 d

Details on results:

pH during test phase: 6.5 to 7.6
The biotic degradation of the test-substance remained clearly below 70% lying between 30% to 40%. It is therefore considered not to be readily biodegradable.
It cannot be excluded that ultimate biodegradation (inherent biodegradation) takes place.
A fraction of about 20% was eliminated via absorption to the biomass.

Results with reference substance:

Degradation of reference substance is above 80%

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The biotic degradation of the test-substance remained clearly below 70% lying between 30% to 40%. It is therefore considered not to be readily biodegradable.
It cannot be excluded that ultimate degradation (inherent biodegradation) takes place.
A fraction of about 20% was eliminated via absorption to the biomass.

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

read-across from supporting substance (structural analogue or surrogate)

Adequacy of study:

key study

Study period:

1988

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

guideline study

Justification for type of information:

Please refer to Section 13

Reason / purpose for cross-reference:

read-across source

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

Qualifier:

according to guideline

Guideline:

EU Method C.4-B (Determination of the "Ready" Biodegradability - Modified OECD Screening Test)

GLP compliance:

yes

Specific details on test material used for the study:

Details on properties of test surrogate or analogue material:
PHYSICO-CHEMICAL PROPERTIES
- Melting point: > 320°C
- Boiling point: NA
- Vapour pressure: NA
- Henry's law constant (for volatie substances): NA
- Water solubility (under test conditions): ca 171 g/l (corrected for purity)
- Solubility in organic solvents: NA
- log Pow: ca. 7.1

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge, domestic, non-adapted

Details on inoculum:

- Source of inoculum/activated sludge: Kommunale Kläranlage Frankfurt-Sindlingen
- Preparation of inoculum for exposure:
- Pretreatment: according to directive section 1.6.3.1
- Concentration of sludge: according to directive section 1.6.4

Duration of test (contact time):

28 d

Initial conc.:

110 mg/L

Based on:

test mat.

Initial conc.:

39.02 mg/L

Based on:

DOC

Parameter followed for biodegradation estimation:

DOC removal

Parameter:

% degradation (DOC removal)

Value:

30 - 40

Sampling time:

28 d

Details on results:

pH during test phase: 6.5 to 7.6
The biotic degradation of the test-substance remained clearly below 70% lying between 30% to 40%. It is therefore considered not to be readily biodegradable.
It cannot be excluded that ultimate biodegradation (inherent biodegradation) takes place.
A fraction of about 20% was eliminated via absorption to the biomass.

Results with reference substance:

Degradation of reference substance is above 80%

Validity criteria fulfilled:

yes

Interpretation of results:

under test conditions no biodegradation observed

Conclusions:

The biotic degradation of the test-substance remained clearly below 70% lying between 30% to 40%. It is therefore considered not to be readily biodegradable.
It cannot be excluded that ultimate degradation (inherent biodegradation) takes place.
A fraction of about 20% was eliminated via absorption to the biomass.

Description of key information

The biotic degradation of the test-substance remained clearly below 70% lying between 30% to 40%. It is therefore considered not to be readily biodegradable.
It cannot be excluded that ultimate biodegradation (inherent biodegradation) takes place.
A fraction of about 20% was eliminated via absorption to the biomass.

Key value for chemical safety assessment

Biodegradation in water:

not biodegradable

Type of water:

freshwater