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Diss Factsheets

Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Administrative data

Link to relevant study record(s)

Description of key information

OECD 207: 
- LC50(14-d): >1000 mg/kg dw test mat. (nominal) based on: mortality;
- NOEC (14-d): ≥1000 mg/kg dw test mat. (nominal) based on: mortality.
OECD 222: OECD (28-d): ≥1000 mg/kg dw test mat. (nominal) based on: survival and reproduction.

Key value for chemical safety assessment

Additional information

An acute earthworm toxicity study is available for GTL Gasoil (Priestly, 2010b). The test was conducted in accordance with OECD 207 and GLP, however no analytical monitoring of the test substance in the exposure medium was carried out. Earthworms (Eisenia foetida) were exposed to a single concentration of GTL Gasoil at a nominal loading rate of 1000 mg/kg of soil (dry weight) for a period of 14 days. There were no significant mortalities or sub-lethal effects observed during the 14-day exposure period.

The 14-d acute LC50for earthworms was therefore determined to be greater than 1000 mg/kg dw.


In addition, an earthworm reproduction study, conducted in accordance with OECD 222 and GLP has also been performed with GTL Gasoil (Harlan, 2011c). No analytical monitoring was carried out. Adult earthworms were exposed in treated soils for a period of four weeks, at test concentrations of 10, 32, 100, 320, and 1000 mg per kg dry soil. Additionally, a control and a reference item treatment were tested in parallel. After this period, the adults were removed from the test vessels and the survival and growth rate were determined. The cocoons and juvenile earthworms remained in the vessels for additional four weeks. The reproduction rate was determined by counting the number of offspring hatched from the cocoons after this additional test period of four weeks. The NOEC of this study based on survival, growth and reproduction parameters was at least 1000 mg/kg dw. A LOEC could not be established due to the absence of adverse effects at the highest dose level.


The following information is taken into account for toxicity to terrestrial macroorganisms:

(28-d) NOEC ≥1000 mg/kg dwE. foetida.

Value used for CSA:

- Short-term LC50for soil macroorganisms: >1000 mg/kg dw;

- Long-term NOEC for soil macroorganisms: ≥1000 mg/kg dw.