α-chlorotoluene

Toxicity to microorganisms

Administrative data

Endpoint:

toxicity to microorganisms

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

The authors performed a cell multiplication inhibition test to assess if benzylchloride is hazardous to the bacteria Pseudomonas putida. The methodology is explained with great detail and the study is generally well described. Thus we consider this a Klimisch 2e study as the study is well documented, meets generally accepted scientific principles and is acceptable for assessment.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Grenzwerte der Schadwirkung wassergefährdender Stoffe gegen Bakterien (Pseudomonas putida) und Grünalgen (Scenedesmus quadricauda) im Zellvermehrungshemmtest

Author:

Bringmann G. and Kühn R.

Year:

1977

Bibliographic source:

Z. f. Wasser- und Abwasser-Forschung, 10(3/4): 87-98

Reference 2

Reference Type:

publication

Title:

Comparison of the toxicity thresholds of water pollutants to bacteria, algae, and protozoa in the cell multiplication inhibition test

Author:

Bringmann G. and Kühn R.

Year:

1980

Bibliographic source:

Water Research, 14: 231-241

Materials and methods

Principles of method if other than guideline:

The authors performed a cell multiplication inhibition test. Using this method the onset of the inhibition of cell multiplication under the influence of the test substance is determined.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

α-chlorotoluene

EC Number:

202-853-6

EC Name:

α-chlorotoluene

Cas Number:

100-44-7

Molecular formula:

C7H7Cl

IUPAC Name:

(chloromethyl)benzene

Constituent 2

Reference substance name:

chloromethylbenzene

IUPAC Name:

chloromethylbenzene

Details on test material:

- Name of test material (as cited in study report): benzylchlorid

No more data available

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

not specified

Test organisms

Test organisms (species):

Pseudomonas putida

Study design

Test type:

static

Water media type:

not specified

Limit test:

no

Total exposure duration:

16 h

Results and discussion

Effect concentrations

Duration:

16 h

Dose descriptor:

other: toxicity threshold

Effect conc.:

4.8 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

growth inhibition

Remarks on result:

other: similar to EC5

Applicant's summary and conclusion

Validity criteria fulfilled:

not applicable

Conclusions:

The authors performed a cell multiplication inhibition test to assess if benzylchloride is hazardous to the bacteria Pseudomonas putida. Under these testing conditions the toxicity threshold (i.e. similar to EC5) of Pseudomonas putida after 16h of exposure was 4.8 mg/L (nominal concentration).

Executive summary:

The authors performed a cell multiplication inhibition test to assess if benzylchloride (CAS n° 100 -44-7) is hazardous to the bacteria Pseudomonas putida. Under static conditions Pseudomonas putida was exposed to a dilution series of the test substance (in triplicate) for 16h at 25°C after which the growth inhibition caused by the test substance was assessed using turbidity measurement comparing the cultures with test substance to a blanc. Under these testing conditions the toxicity threshold (i.e. similar to EC5) of Pseudomonas putida was 4.8 mg/L (nominal concentration).

No data is available on the GLP status and no guideline is followed. However, the methodology used in this study is described with great detail and the study itself meets generally accepted scientific principles and is as such acceptable for assessment. Therefore we consider the study reliable with restrictions (klimisch 2e).