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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: oral
Type of information:
experimental study
Adequacy of study:
key study
Study period:
1966
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Remarks:
Study performed in 1966, probably no GLP, reasonably documented (2-page summary report), but not sufficient to comply with guideline. Probably only one male animal per dose. Necropsy / pathological evaluation omitted. Submitted to US Office of Toxic Substances under TSCA, 1982.

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1966
Report date:
1966

Materials and methods

Test guideline
Qualifier:
no guideline followed
Principles of method if other than guideline:
Individual male animals treated by gavage with a wide range of doses (8 animals, 670 - 25000 mg/kg bw). Time of death and symptoms recorded.
GLP compliance:
no
Test type:
other: Approximate LD50, 1 animal per dose
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Trimethyl orthoformate
EC Number:
205-745-7
EC Name:
Trimethyl orthoformate
Cas Number:
149-73-5
Molecular formula:
C4H10O3
IUPAC Name:
trimethoxymethane
Details on test material:
- Name of test material (as cited in study report): Trimetyl orthoformate TMOF
- Substance type: pure active substance
- Physical state: liquid
- Analytical purity: 98%
- Density: 0.97 g/mL
- Impurities (identity and concentrations): no data
- Used as received

Test animals

Species:
rat
Strain:
other: ChR-CD
Sex:
male

Administration / exposure

Route of administration:
oral: gavage
Vehicle:
arachis oil
Details on oral exposure:
VEHICLE
- Concentration in vehicle: 15% (up to 2250 mg/kg bw), 40% (3400-5000 mg/kg bw), and 100% (7500-25000 mg/kg bw)
- Amount of vehicle (if gavage): Depending on dose
- Purity: no data

MAXIMUM DOSE VOLUME APPLIED:
25 ml/kg bw (pure test substance)

Doses:
670, 2250, 3400, 5000, 7500, 11000, 17000, 25000 mg/kg body weight
No. of animals per sex per dose:
1 male
Control animals:
no
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: no data
- Necropsy of survivors performed: no (at request of the sponsoring department)
- Other examinations performed: clinical signs, body weight
Statistics:
none

Results and discussion

Effect levels
Key result
Sex:
male
Dose descriptor:
approximate LD50
Effect level:
5 000 mg/kg bw
Based on:
test mat.
Remarks on result:
other: Death at 5000 mg/kg bw occurred 1 day after application; no deaths below this dose.
Mortality:
Death of single animal exposed: within minutes at doses above 7500 mg/kg bw; after one day upon treatment with 5000 mg/kg bw; no deaths below this dose
Clinical signs:
other: Lethal doses: incoordiantion, cyanosis, loss of muscle tone, rapid respiration, prostration, unresponsiveness Non-lethal doses: decreased muscle tone for two hours post-treatment

Applicant's summary and conclusion

Interpretation of results:
GHS criteria not met