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EC number: 276-743-1 | CAS number: 72624-02-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1993-07-02 to 1993-08-02
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: see 'Remark'
- Remarks:
- 2-Aminoanthracene was used as a positive control for all stains with S9 (OECD 471 notes that 2-AA should not be used as the sole indicator of the efficacy of the S-9 mix), however, the S9 mix was evaluated with other positive controls prior to testing (non-concurrent). Not all of the tests had 5 concentrations that were not cytotoxic, however, there was no indication that an increase in revertant colonies would have been seen even if more non-cytotoxic doses had been included.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 993
- Report date:
- 1993
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- Deviations:
- yes
- Remarks:
- Minor deviations relating to positive control used with-S9 and the number of non-toxic dose levels
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Phenol, heptyl derivs.
- EC Number:
- 276-743-1
- EC Name:
- Phenol, heptyl derivs.
- Cas Number:
- 72624-02-3
- Molecular formula:
- C13H20O
- IUPAC Name:
- 4-(2-methylhexyl)phenol; 4-heptylphenol
- Details on test material:
- - Physical state: amber visous liquid
- Storage condition of test material: room temperature
Constituent 1
Method
- Target gene:
- Histidine locus in Salmonella, tryptophan locus in E.coli
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- S. typhimurium TA 1538
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- Aroclor S9
- Test concentrations with justification for top dose:
- 0, 0.5, 1.67, 5.0, 16.7, 50.0, 167 ug/plate in the preliminary test for all strains
0, 0.05, 0.167, 0.5, 1.67, 5.0, 16.7 ug/plate in test 1 for all salmonella strains
0, 0.167, 0.5, 1.67, 5.0, 16.7, 50.0 ug/ml in test 1 for E.coli
0, 0.05, 0.167, 0.5, 1.67, 5.0, 16.7 ug/plate in a confirmatory test salmonella strain TA1538
0, 1.67, 5.0, 16.7, 50.0, 167, 500 ug/plate in a confirmatory test for the other salmonella strains and E.coli
0, 0.5, 1.67, 5.0, 16.7, 50.0, 100 ug/plate in a third test in salmonella strains TA1535, TA1537, TA98 and TA100 with S9 only
0, 0.167, 0.5. 1.67, 5.0, 16.7, 50.0, 100 ug/plate in a third test in E.coli with S9 only - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: DMSO
- Justification for choice of solvent/vehicle: The test article was soluble in DMSO but insoluble in water
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- sodium azide
- Remarks:
- without S9
Migrated to IUCLID6: 10.0 ug/plate TA100 and TA1535
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 9-aminoacridine
- Remarks:
- without S9
Migrated to IUCLID6: 150 ug/plate TA1537
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- 2-nitrofluorene
- Remarks:
- without S9
Migrated to IUCLID6: 5.0 ug/plate TA98 and TA1538
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- N-ethyl-N-nitro-N-nitrosoguanidine
- Remarks:
- without S9
Migrated to IUCLID6: 2.0 ug/plate E.coli WP2 uvrA
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- other: 2-anthramine 2.5 ug/plate for Salmonella, 80 ug/plate for E. coli
- Remarks:
- with S9
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: preincubation for 30 minutes prior to plating
DURATION
- Preincubation period: 30 minutes
- Exposure duration: 48 hours
NUMBER OF REPLICATIONS: triplicate plates per dose level
NUMBER OF CELLS EVALUATED: Approximately 1 x 10E8 cells per plate
DETERMINATION OF CYTOTOXICITY
- Method: Evaluation of agar plates for reduced growth of the background lawn, pindot colnies - Evaluation criteria:
- A positive result is defined as a statistically significant, dose-dependent increase in the number of histidine- or tryptophan-independent revertants with at least one dose level inducing a revertant frequency that is two-fold the spontaneous solvent control value. Statistical analyses were performed using the program developed by Snee and Irr (1981), with significance estalished at the 95% confidence limit. If the test article does not induce a statistically significant, dose-dependent increase in revertant frequency, but does induce a revertant frequency at one dose level that is two-fold the spontaneous control value, the result is considered equivocal. A negative result is defined as the absence of a statistically significant or dose-dependent increase in the number of histidine- or tryptophan-independent revertants.
- Statistics:
- Snee RD and JD Irr (1981) Design of a statistical method for the analysis of mutagenesis at the hypoxanthine guanine phosphoribosyl transferase locus of cultured Chinese hamster ovary cells, Mutation res., 85:77-93.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- S. typhimurium TA 1538
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- In an initial preliminary toxicity test the test material precipitated at 500, 1670 and 5000 ug/plate but these were also toxic dose levels.
- Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
The data tables from the study report are attached as a Word file. For some strains in some tests, either with or without S9, there were fewer than four dose levels without toxicity. However, in most cases the toxicity was limited to a modest effect on the growth of the background lawn of bacteria and the frequency of revertant colonies was not affected.
2-Aminoanthracene was used as a positive control for all strains with S9, whereas OECD 471 notes that 2-AA should not be used as the sole indicator of the efficacy of the S-9 mix, however, the S9 mix was evaluated with other positive controls prior to testing (non-concurrent), which provides confidence in the results with-S9.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative
The results for the test item were negative in the Ames/Salmonell-E.coli Liquid Pre-incubation assay under the conditions, and according to the criteria, of the test protocol. - Executive summary:
Test Guidance
OECD Guideline 471
Method and Materials
The test item was evaluated in the Ames/Salmonella-E.coli liquid Pre-incubation assay to determine its ability to induce reverse mutations at selected histidine loci in five tester strains of Salmonella (TA1535, TA1537, TA1538, TA98 and TA100) and at the tryptophan locus in one Escherichia coli strain (WP2 uvrA), in the presence and absence of an exogenous metabolic activation system (S9). Toxicity of the test material was initially evaluated in a prescreen by treating duplicate cultures of strains TA1538, TA100 and WP2 uvrA with the test article at doses of 50, 167, 500, 1670 and 5000 ug/plate in the absence of S9. Results of the pre-screen indicated the test material produced inhibited growth (characterised by a reduced background lawn and/or the presence of pindot colonies) or complete toxicity in all three tester strains at all doses evaluated. In addition, the test article was incompletely soluble at dose >= 500 ug/plate. Therefore, the test item was re-evaluated under identical conditions at doses of 0.5, 1.67, 5.0, 16.7, 50 and 167 ug/plate. The test item again produced inhibited growth or complete toxicity in strains TA1538 and TA100 at dose >= 5.0 ug/plate, and in strain WP2 uvrA at doses >= 16.7 ug/plate.
Based upon these findings, the test item was evaluated in triplicate cultures in all five Salmonella strains at doses of 0.05, 0.167, 0.5, 1.67, 5.0, 16.7 ug/plate, and in strain WP2 uvrA at doses of 0.167, 0.5, 0.67, 5.0, 16.7 and 50 ug/plate, in the presence and absence of S9. A further confirmatory test using modified dose ranges was performed both in the presence and absence of S9 and a third test was performed with all strains in the presence of S9 only.
Results
Toxicity was observed that varied both between strains and between the exposure conditions in the presence and absence of S9. However, overall a satisfactory number of analysable dose levels was achieved in most cases, both in the presence and absence of S9. There were no statistically significant and dose-related increases in the frequency of revertant colonies seen for any strain of bacteria, either in the presence or absence of S9.
Conclusion
Therefore, the results for the test item were negative in the Ames/Salmonella-E.coli Liquid Pre-incubation assay under the conditions, and according to the criteria, of the test protocol.
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