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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Administrative data

Endpoint:
acute toxicity: dermal
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)

Data source

Reference
Reference Type:
study report
Title:
Unnamed
Year:
1971
Report date:
1971

Materials and methods

Test guideline
Qualifier:
equivalent or similar to guideline
Guideline:
EU Method B.3 (Acute Toxicity (Dermal))
GLP compliance:
not specified
Test type:
fixed dose procedure
Limit test:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
(E)-anethole
EC Number:
224-052-0
EC Name:
(E)-anethole
Cas Number:
4180-23-8
Molecular formula:
C10H12O
IUPAC Name:
1-methoxy-4-prop-1-en-1-ylbenzene

Test animals

Species:
rabbit
Strain:
New Zealand White
Sex:
male/female
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Weight at study initiation: 2.5-3.0 kg
- Housing: Individually housed
- Diet: commercial diet
- Water: ad libitum

Administration / exposure

Type of coverage:
semiocclusive
Vehicle:
unchanged (no vehicle)
Details on dermal exposure:
TEST SITE
- Area of exposure: 240 cm2
- % coverage: 10%
- Type of wrap if used: Rubber sleeve and Webril padding

REMOVAL OF TEST SUBSTANCE
- Time after start of exposure: 24h

TEST MATERIAL
- Amount(s) applied (volume or weight with unit): 4900 mg/kg
- Constant volume or concentration used: yes/no
Duration of exposure:
24h
Doses:
4900 mg/kg bw
No. of animals per sex per dose:
6 (male/female)
Control animals:
not specified
Details on study design:
- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: daily
- Necropsy of survivors performed: yes
Statistics:
no data

Results and discussion

Effect levels
Dose descriptor:
LD50
Effect level:
> 4 900 mg/kg bw
Based on:
test mat.
Mortality:
No deaths.
Clinical signs:
other: Edema (3 of 6 rabbits), Erythema (6/6), Dicoloration (-), Scaling (6/6), Necrosis (-) and Eschar formation (2/6)
Gross pathology:
There was no tissue damage observed in rabbits killed at the termination of the study.
Other findings:
No data

Any other information on results incl. tables

The acute dermal LD50 value appear to be greater than 4900 mg/kg bw. No toxic signs were observed in rabbits receiving test material during the 14 -day observation period.

Applicant's summary and conclusion

Interpretation of results:
not classified
Remarks:
Migrated information Criteria used for interpretation of results: EU
Conclusions:
Anethole is not classified according to Regulation (EC) No 1272/2008
Executive summary:

Anethole was screened for acute dermal toxicity in rabbits. Test material (4900 mg/kg bw) produced no deaths, it is assumed that the acute dermal LD50 value is above 4900 mg/kg bw in rabbits.