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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Endpoint summary

Administrative data

Description of key information

Additional information

Short-term Toxicity to Fish

This study was carried out to determine the toxicity of Cycloaliphatic Eepoxide resin ERL-4221 to rainbow trout. The study was carried out as a flow-through test in accordance with OECD Guideline 203. The test species were exposed to the following nominal test concentrations: Dilution water control, solvent control, 1.8, 3.2, 5.6, 10, 18 and 32 mg/L, mean measured exposure concentrations were 2.7, 2.9, 5.4, 10, 19 and 31 mg/L. Mortality was only observed in the 32 mg/L treatment where all of the fish had died after 72 hours exposure. Based on the nominal exposure concentrations the following results were obtained:

The 96-hour LC50: 24 mg/L

The 96-hour LC100: 32 mg/L

The NOEC (96 -hour): 18 mg/L (based on mortality) or 3.2 mg/L based on sub-lethal effects.

Short-term Toxicity to Aquatic Invertebrate

This study was conducted to determine the toxicity of Cycloaliphatic Epoxide resin ERL-4221 to Daphnia magna. This study was carried out under static test conditions in accordance with OECD Guideline 202. The test species were exposed to the following nominal test concentrations: Dilution water control and nominal 5.6, 10, 18, 32, 56, 100 and 180 mg/L, mean measured exposure concentrations were 5.2, 9.4, 16, 28, 57, 101 and 160 mg/L. 

After 48 hours, at the nominal concentration 32 mg/L and 56 mg/L observed immobility was 50 and 55% respectively, while at the higher concentrations of 100 mg/L and 180 mg/L, immobility was 5 and 20 % respectively. Therefore, to obtain a meaningful EC50 value, the 48 hour EC50 was calculated by exclusion of the highest two concentrations. However, the effects observed in this study do not satisfy the established criteria of a maximum effect of 65 % for calculation of an EC50.

 

Despite this, a reasonable judgement can be made from the data that the 48 hour EC50 value lies somewhere between 32 and 56 mg/L (where observed effects were 50 and 55 % respectively, so the value calculated using Stephan's method of 40 mg/L is appropriate.


The NOEC (48-hour) was observed to be 10 mg/L.

Toxicity to Aquatic Algae and Cyanobacteria

This study was conducted to determine the toxicity of Cycloaliphatic Epoxide resin ERL-4221 to the green algae Selenastrum capricornutum. This study was carried out in accordance with OECD Guideline 201. The study was performed with the following nominal test concentrations: control, 5.6, 10, 18, 32, 56, 100 and 180 mg/L, mean measured exposure concentrations were 3.7, 6.6, 11, 22, 30, 27 and 110 mg/L. The results of the study are based on the mean measured concentrations.

The 72 hour EbC50was calculated to be 90 mg/L, the corresponding lowest observed effect concentration (LOEC) and no-observed effect concentration (NOEC) were calculated to be 27 and 22 mg/L. The calculated NOEC and LOEC are lower than indicated by the areas under the growth curve and therefore present a worst case result. This is due to the inclusion of the low geometric mean measured concentration for the nominal 100 mg/L concentration in the statistical analysis.

The 72 hour ErC50was observed to be >110 mg/L, the highest mean measured concentration tested. The corresponding NOEC was 30 mg/L. 

Toxicity to microorganisms

The impact of Celloxide 2021P on the respiration rate of activated sludge was assessed according to the OECD Guideline 209 (Adopted 1984). Samples of activated sludge were exposed, for three hours, to a range of concentrations of Celloxide 2021P. Each preparation contained dechlorinated tap water and a synthetic sewage preparation that provided a uniform respiration substrate. At the end of the exposure period, the respiration rate of the activated sludge microbes was determined by measuring the decline in oxygen concentration of the test culture. The inhibitory effect of the test substance was calculated by comparing the respiration rate of cultures containing test substance to that of blank control cultures containing only activated sludge, dechlorinated tap water and synthetic sewage. A series of cultures containing a reference substance (3,5‑dichlorophenol) confirmed that the sensitivity of the sludge was within acceptable limits. In the definitive test, activated sludge was exposed to Celloxide 2021P at nominal concentrations of 19, 61, 195, 625 and 2000 mg/L. The mean respiration rates observed at each of the test concentrations were 71, 71, 68, 57 and 49 mg O2/L/h respectively. The mean respiration rates for the 19, 61 and 195 mg/L test concentrations were within the range observed for the blank control samples (67 to 75 mgO2/L/h) suggesting no inhibitory effect. However, at the 625 and 2000 mg/L test concentrations, an inhibitory effect of 19% and 31% respectively was observed. Based on Probit analysis, the effective concentrations of Celloxide 2021P that caused a 10% and 20% reduction in respiration rate relative to the untreated controls (EC10and EC20) were 409 and 917 mg/L respectively. The EC50 and EC80 could not be reliably determined but can be said to be >2000 mg/L. The no observed effect concentration (NOEC) is considered to be 195 mg/L.