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Diss Factsheets

Ecotoxicological information

Toxicity to aquatic algae and cyanobacteria

Administrative data

toxicity to aquatic algae and cyanobacteria
Type of information:
experimental study
Adequacy of study:
key study
Study period:
12-15 Novemer 2002
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Study conducted in compliance with agreed protocols, with no or minor deviations from standard test guidelines and/or minor methodological deficiencies, which do not affect the quality of the relevant results. Study was in compliance with OECD Good Laboratory Practice (GLP). Derivations were involved in obtaining the toxicity values using statistical model.

Data source

Reference Type:
study report
Report date:

Materials and methods

Test guideline
according to guideline
OECD Guideline 201 (Alga, Growth Inhibition Test)
not specified
GLP compliance:

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
- Name of test material (as cited in study report): Trichlorooctylstannane
- Molecular formula (if other than submission substance): C8Hl7CI3Sn
- Molecular weight (if other than submission substance): 338.3 g/mol
- Physical state: colourless liquid
- Analytical purity: 100.0% n-octyltin trichloride
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material (migrated information):
No information

Sampling and analysis

Analytical monitoring:
Details on sampling:
At the start of the growh inhibition test, duplicate 100 mL samples were taken from all media (without algae) containing all nominal test substance concentrations tested, together with a control. At the end of the test, duplicate 100 mL samples were taken from the media containing the same test substance concentrations with algae. One of the duplicate sample series at the start and at the end of the test was analysed. The remaining samples were stored in a freezer to allow later analysis.

Test solutions

not specified
Details on test solutions:
The test substance was added directly to an appropriate quantity of algal medium in an Erlenmeyer flask, followed by stirring for 16-20 hours using a magnetic stirrer. The stirring was such that the vortex created extended to ca. 1/3 of the dept of the fluid column. Following stirring, the medium was allowed to stand for ca. 4 hours to enable phase separation. The clear aqueous phase was tapped and diluted to achieve a concentration series. The first 100 mL was discarded. The saturated solutions were diluted with algal medium to prepare the test dilutions.

Test organisms

Test organisms (species):
other: green alga (Scenedesmus subspicatus (SAG 86.81)
Details on test organisms:
The freshwater green alga Scenedesmus subspicatus (SAG 86.81), which belongs to the order of Chlorococcales (class Chlorophyceae), was used as the test organism. The culture was supplied by the Collection of Algal Cultures, Institue for Plant Phsiology, University of Gottingen, Nikolausberger Weg 18, D-37073 Gottingen, Germany. A pre-culture of algae in the exponential growth phase was prepared as detailed in OECD Guideline no. 201, using hte medium described in 2.4. The initial inoculum cell density was 0.3 x 10^4 cells/mL to obtain the appropriate biomass level in the test medium.

Study design

Test type:
Water media type:
Limit test:
Total exposure duration:
72 h
Post exposure observation period:
No information.

Test conditions

No information.
Test temperature:
21.7 - 23.6 ºC.
Ranged from 7.5 to 8.0.
Dissolved oxygen:
No information.
No information.
Nominal and measured concentrations:
No information.
Details on test conditions:
Light intensity 81 - 100 µmols/s/m^2.
Reference substance (positive control):
not specified

Results and discussion

Effect concentrationsopen allclose all
72 h
Dose descriptor:
Effect conc.:
0.045 mg/L
Basis for effect:
growth rate
72 h
Dose descriptor:
Effect conc.:
0.215 mg/L
Basis for effect:
growth rate
Details on results:
The study meet validty criteria in OECD test guideline 201.
Results with reference substance (positive control):
No information.
Reported statistics and error estimates:
The toxicity estimates derived from the statistical model demonstrated that MOTC had a significant adverse effect on the growth rate. The lower EbC50 value is therefore considered to have no toxicological relevance.

Any other information on results incl. tables

No other information.

Applicant's summary and conclusion

Validity criteria fulfilled:
The 72-h toxicity of trichlorooctylstannane to fresh water green alga (Scenedesmus subspicatus) (ErC50) was 0.215 mg/L. The NOEC was 0.045 mg/L.
Executive summary:

The toxicity of trichlorooctylstannane to fresh water green alga (Scenedesmus subspicatus) was determined in a 72 -h growth inhibition test according to OECD Guideline 201 (Alga, Growth Inhibition Test) and in compliance with the OECD principles of Good Laboratory Practices (GLP). The ErC50 was determined to be 0.215 mg/L based on the average measured concentrations of the test substance. The NOEC was 0.045 mg/L.