Registration Dossier
Registration Dossier
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EC number: 254-274-3 | CAS number: 39072-70-3
Biodegradation in water: screening tests
Administrative data
- Endpoint:
- biodegradation in water: ready biodegradability
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)
- GLP compliance:
- yes
Test material
- Reference substance name:
- N,N''-hexane-1,6-diylbis[N'-benzylurea]
- EC Number:
- 254-274-3
- EC Name:
- N,N''-hexane-1,6-diylbis[N'-benzylurea]
- Cas Number:
- 39072-70-3
- Molecular formula:
- C22H30N4O2
- IUPAC Name:
- N',N'''-hexane-1,6-diylbis(1-benzylurea)
- Details on test material:
- - Name of test material (as cited in study report): N,N''-Hexane-1,6-diylbis[N'-benzylurea]
- Physical state: Solid / white
- Analytical purity: 100%
- Lot/batch No.: 259-635
- Storage condition of test material: Storage at room temperature, no direct sunlight, protect
against humidity
Constituent 1
Study design
- Oxygen conditions:
- aerobic
- Inoculum or test system:
- activated sludge, domestic, non-adapted
- Details on inoculum:
- - Source of inoculum/activated sludge (e.g. location, sampling depth, contamination history, procedure): Laboratory wastewater plants treating municipal and synthetic sewage
- Preparation of inoculum for exposure: Inoculum was pre-aerated two days before the start of the test
- Initial cell/biomass concentration: 30 mg/L dry substance - Duration of test (contact time):
- 28 d
Initial test substance concentrationopen allclose all
- Initial conc.:
- 29 mg/L
- Based on:
- test mat.
- Initial conc.:
- 20 mg/L
- Based on:
- DOC
Parameter followed for biodegradation estimation
- Parameter followed for biodegradation estimation:
- CO2 evolution
- Details on study design:
- TEST CONDITIONS
- Test temperature: 22 ± 2 °C
TEST SYSTEM
- Number of culture flasks/concentration: 2
- Method used to create aerobic conditions: pre-aeration for two days before test start
- Measuring equipment: carbon dioxide trap
- Test performed in open system: no
SAMPLING
- Sampling frequency: every other day
CONTROL AND BLANK SYSTEM
- Inoculum blank: yes
- Abiotic sterile control: no
- Other: Inhibition control
Results and discussion
% Degradation
- Parameter:
- % degradation (CO2 evolution)
- Value:
- < 10
- Sampling time:
- 28 d
BOD5 / COD results
- Results with reference substance:
- 81% after 28 days
Applicant's summary and conclusion
- Interpretation of results:
- other: not readily biodegradable under the conditions of this test
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