Diisopropyl succinate

Short-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

short-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

key study

Study period:

10 January 2002 to 30 January 2002

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Test procedures according to national standard, no GLP, no chemical analysis and moderate documentation.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: ÖNORM EN ISO 6341

Deviations:

no

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

Diisopropyl succinate

EC Number:

213-110-0

EC Name:

Diisopropyl succinate

Cas Number:

924-88-9

Molecular formula:

C10H18O4

IUPAC Name:

diisopropyl succinate

Details on test material:

- Name of test material: Diisopropylsuccinat
- Amount: ca. 50 mL
- Storage: at 4°C until the investigation

Sampling and analysis

Analytical monitoring:

no

Test solutions

Details on test solutions:

PREPARATION AND APPLICATION OF TEST SOLUTION
- Method: The test substance was dilluted in deionised water (Millipore ELIX 10) immediately before the start of the test. The pH value was adjusted with NaOH or H2SO4 to 7.8 +- 0.2.
- Evidence of undissolved material (e.g. precipitate, surface film, etc): no.

Test organisms

Test organisms (species):

Daphnia magna

Details on test organisms:

TEST ORGANISM
- Common name: Daphnia magna STRAUS (HFA-stem C1)
- Age at study initiation: 1 to 24 hours

Study design

Test type:

static

Water media type:

freshwater

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

no

Test conditions

Hardness:

no data

Test temperature:

20 +- 2°C

pH:

the pH value was adjusted with NaOH or H2SO4 to 7.8 +- 0.2.

Dissolved oxygen:

no data

Salinity:

not applicable.

Nominal and measured concentrations:

Nominal concentrations: 0; 0.2; 0.3 and 0.4 mL/L corresponding to (density of the test substance = 0.9847 g/cm3) 0.0; 197; 295 and 394 mg/L in the main experiment and 0; 0.1; 0.5 and 1.0 mL/L corresponding to (density of the test substance = 0.9847 g/cm3) 0.0; 98; 492 and 985 mg/L in a preliminary experiment.

Details on test conditions:

TEST SYSTEM
- Test vessel: no data
- No. of vessels per concentration (replicates): 4

TEST MEDIUM / WATER PARAMETERS
- no data

OTHER TEST CONDITIONS
- Adjustment of pH: yes
- Photoperiod: The test was conducted in the dark.

EFFECT PARAMETERS MEASURED :Immobilisation % after 24 hours

TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2
- Range finding study: A preliminary study using nominal concentrations of 0; 98; 492 and 985 mg/L was conducted and 0; 0; 100; and 100 % immobilisation was observed respectively. The results of the preliminary study were used for determination of the concentrations for the main experiment as well as for the determination of the EC50 of the whole investigation.

Reference substance (positive control):

not specified

Results and discussion

Effect concentrations

Effect concentrations 1

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

> 100 mg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Effect concentrations 2

Duration:

24 h

Dose descriptor:

EC50

Effect conc.:

ca. 0.3 other: mL/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

mobility

Details on results:

For immobilisation see table 1.
- Behavioural abnormalities: not stated
- Mortality of control: 0%
- Other adverse effects control: not stated
- Abnormal responses: not stated
- Any observations (e.g. precipitation) that might cause a difference between measured and nominal values: no

Results with reference substance (positive control):

no data

Reported statistics and error estimates:

no data

Any other information on results incl. tables

Table 1: Immobilisation

Test substance concentration (mg/L)	0	98	197	295	394	492	985
immobilisation 24 hours in %, preliminary study	0	0	not tested	not tested	not tested	100	100
immobilisation 24 hours in %, main study	0	not tested	70	65	95	not tested	not tested

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Conclusions:

EC50 24 hours > nominal 100 mg/L

Executive summary:

The immobilisation data from the preliminary test and the main experiment were used for the determination of the EC50 24 hours. The EC50 24 hours is nominal greater than 100 mg/L.