Bis(2,6-diisopropylphenyl)carbodiimide

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Study period:

1962

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

other: very old report only minimal information given

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Unnamed

Year:

1962

Report date:

1962

Reference 2

Reference Type:

other:

Title:

Unnamed

Year:

1979

Report date:

1979

Materials and methods

Test guideline

Qualifier:

no guideline followed

Principles of method if other than guideline:

Carbodiimide was administered in an oily solution to male rats via intraperitoneal injection. The animals were observed for at least 5 days and clinical signs and mortality monitored.

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Bis(2,6-diisopropylphenyl)carbodiimide

EC Number:

218-487-5

EC Name:

Bis(2,6-diisopropylphenyl)carbodiimide

Cas Number:

2162-74-5

Molecular formula:

C25H34N2

IUPAC Name:

N,N'-bis[2,6-bis(propan-2-yl)phenyl]methanediimine

Details on test material:

- Name of test material (as cited in study report): Carbodiimid 4175
- Physical state: solid (bright yellow to brown and practically odour-less crystalline substance)

Test animals

Species:

rat

Strain:

other: Albino, unspecified strain

Sex:

male

Details on test animals or test system and environmental conditions:

no further details available

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

other: oil, unspecified

Details on exposure:

no data

Doses:

0.01 g/kg (0.2% in oil)
0.025 g/kg (0.5% in oil)
0,05 g/kg (0.5% in oil)
0.10 g/kg (1% in oil)
0.25 g/kg (5% in oil)
0.5 g/kg (10% in oil)

No. of animals per sex per dose:

5 (male rats only)

Control animals:

not specified

Details on study design:

no further details

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LC0

Effect level:

>= 0.01 mg/kg bw

Based on:

test mat.

Effect levels 2

Sex:

male

Dose descriptor:

LC50

Effect level:

ca. 0.2 mg/kg bw

Based on:

test mat.

Effect levels 3

Sex:

male

Dose descriptor:

LC100

Effect level:

0.5 mg/kg bw

Based on:

test mat.

Mortality:

0.01 g/kg: no mortality
0.025 g/kg: no mortality
0.05 g/kg: no mortality
0.1 g/kg: no mortality
0.25 g/kg: 4 dead rats
0.50 g/kg: 5 dead rats

Clinical signs:

0.01 g/kg: no clinical signs
0.025 g/kg: 5 animals with symptoms
0.05 g/kg: 5 animals with symptoms
0.10 g/kg: 5 animals with symptoms
0.25 g/kg: 5 animals with symptoms
0.50 g/kg: 5 animals with symptoms

Symptoms were similar as the symptoms which occurred after oral exposure (reduced general condition, spastic posture, expanded abdomen (start 1 hour after application, reversible within 3 days)).

Body weight:

no data

Gross pathology:

no data

Other findings:

no further details available

Applicant's summary and conclusion

Conclusions:

The study was considered to be of low reliability (reliability Klimisch 3), because of the limited information available. The test material did induce mortality and treatment-related clinical signs in the dose group of 500 mg/kg bw (100 % mortality) and in dose group 250 mg/kg bw (80 % mortality). In the dose groups 10, 25, 50 and 100 mg/kg no mortality was evident.

Executive summary:

The acute intraperitoneal toxicity of bis(2,6-diisopropylphenyl)-carbodiimide was investigated in rats (Kimmerle, 1962). The test item was administered in oil in concentrations of 10, 25, 50, 100, 250 and 500 mg/kg bw via intraperitoneal injection to male albino rats (groups of 5). Mortality occurred with all animals in the group dosed with 500 mg/kg bw. The symptoms were poor general condition, hunched posture and expanded abdomen. 4 animals died in the group dosed with 250 mg/kg bw. No mortality was found in the dose groups of 10, 25, 50 and 100 mg/kg bw. An LD50 of ca. 200 mg/kg bw was identified.