Acrylic acid, monoester with propane-1,2-diol

Acute Toxicity: other routes

Administrative data

Endpoint:

acute toxicity: other routes

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Study meets generally accepted scientific standards with restrictions due to the limited documentation in the summary report

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1974

Report date:

1974

Reference 2

Reference Type:

other: company data

Title:

Unnamed

Year:

2002

Report date:

2002

Materials and methods

Principles of method if other than guideline:

BASF-Test

Aqueous preparations of the test substance were injected into the peritoneal cavity of the mice. Group-wise documentation of clinical signs was performed over the 7-day study period. Body weight was determined before the start of the study only, as it was needed for determination of dose. The clinical signs and findings were reported in summary form

GLP compliance:

no

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Acrylic acid, monoester with propane-1,2-diol

EC Number:

247-118-0

EC Name:

Acrylic acid, monoester with propane-1,2-diol

Cas Number:

25584-83-2

Molecular formula:

C6H10O3

IUPAC Name:

Reaction mass of 2-hydroxy-1-methylethyl acrylate and 2-hydroxypropyl acrylate

Details on test material:

- Name of test material (as cited in study report): Hydroxypropyl acrylate
- Analytical purity: approx. 99 %
- Composition of test material, percentage of components: 66 % 2-Hydroxy-1-propyl acrylate, 33 % 1-Hydroxy-2-propyl acrylate, approx. 1 % free acrylic acid

Test animals

Species:

mouse

Strain:

NMRI

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: no data
- Mean weight at study initiation: 21.6 g (males and females)

Administration / exposure

Route of administration:

intraperitoneal

Vehicle:

water

Details on exposure:

VEHICLE
- Concentration in vehicle: 2, 4, 8, and 16 % (v/v)


MAXIMUM DOSE VOLUME APPLIED: 0.26 mL/animal

Doses:

200, 400, 500, 800, and 1600 µL/kg bw (corresponding to 210.8, 421.6, 527.0, 843.2, and 1686.4 mg/kg bw)
Recalculation based on density = 1.054 g/mL at 20 °C

No. of animals per sex per dose:

5

Control animals:

no

Details on study design:

- Duration of observation period following administration: 7 days
Cageside observations were performed several times on the day of administration and daily on workdays during observation period. Body weights were determined at test start and termination.
- Necropsy of survivors performed: yes
- Other examinations performed: clinical signs, body weight, gross-pathological investigation

Statistics:

On the basis of the observed lethality, the LD50 value was estimated or determined using a graphical evaluation of the dose response curve on probability paper.

Results and discussion

Effect levels

Sex:

male/female

Dose descriptor:

LD50

Effect level:

ca. 474 mg/kg bw

Mortality:

At the highest dose, all animals died within 24 hrs after administration. At 843.2 and 527.0 mg/kg bw 8/10, and 6/10 animals died within 24, and 48 hr, respectively. At the two lower doses, no mortality was observed.

Clinical signs:

The clinical signs observed were dyspnoea, saltatory spasms, tremor, twitching, staggering, lateral-abdominal position, slight atony, apathy, in some cases salivation (described as watery secretion from the oral cavity), narcotic-like state, eyelids glued together up to complete eyelid closure.

Body weight:

Normal body weight development

Gross pathology:

Deceased and sacrificed animals:
No abnormalities observed.

Any other information on results incl. tables

Original value: LGD50 = approx. 450 µL/kg bw

Recalculation based on density = 1.054 g/mL at 20 °C

Mortality:

 

Dose [mg/kg bw]	Conc. [%]	No. of animals/sex	Dead animals / treated animals after
			1 h	24 h	48 h	7 d
1686.4	16	5 m	0/5	5/5	5/5	5/5
		5 f	0/5	5/5	5/5	5/5
843.2	8	5 m	0/5	3/5	3/5	3/5
		5 f	0/5	5/5	5/5	5/5
527.0	8	5 m	0/5	0/5	3/5	3/5
		5 f	0/5	2/5	3/5	3/5
421.6	4	5 m	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5
210.8	2	5 m	0/5	0/5	0/5	0/5
		5 f	0/5	0/5	0/5	0/5

m: male

f: female